50948

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 71827..72353 Replicon chromosome
Accession NZ_CP010160
Organism Escherichia coli strain H1

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag RG44_RS00400 Protein ID WP_000323025.1
Coordinates 71827..72114 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag RG44_RS00405 Protein ID WP_000534858.1
Coordinates 72114..72353 (-) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RG44_RS00355 67182..67913 - 732 WP_073544559.1 helix-turn-helix domain-containing protein -
RG44_RS00365 68320..69279 - 960 WP_000592549.1 DUF523 and DUF1722 domain-containing protein -
RG44_RS00370 69472..69996 + 525 WP_000780584.1 lipocalin family protein -
RG44_RS00375 70152..70529 - 378 WP_001204787.1 antitermination protein -
RG44_RS00380 70547..70753 - 207 Protein_64 DUF968 domain-containing protein -
RG44_RS00385 70787..71065 - 279 WP_001515373.1 hypothetical protein -
RG44_RS00390 71132..71383 - 252 WP_001463433.1 hypothetical protein -
RG44_RS00395 71600..71755 - 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
RG44_RS00400 71827..72114 - 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
RG44_RS00405 72114..72353 - 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
RG44_RS00410 72378..72683 + 306 WP_071593935.1 hypothetical protein -
RG44_RS00420 72886..73218 + 333 WP_001301033.1 protein FlxA -
RG44_RS00425 73655..74995 - 1341 WP_000589013.1 ISNCY family transposase -
RG44_RS00440 75764..76050 - 287 Protein_73 hypothetical protein -
RG44_RS27165 76216..76398 - 183 Protein_74 hypothetical protein -
RG44_RS00450 76661..77017 - 357 WP_001315552.1 class I SAM-dependent methyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 64890..103458 38568


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T50948 WP_000323025.1 NZ_CP010160:c72114-71827 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T50948 NZ_CP010160:c72114-71827 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGATCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT50948 WP_000534858.1 NZ_CP010160:c72353-72114 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT50948 NZ_CP010160:c72353-72114 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
AlphaFold DB A0A4V1CTS8

References