Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 71827..72353 Replicon chromosome
Accession NZ_CP010160
Organism Escherichia coli strain H1

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag RG44_RS00400 Protein ID WP_000323025.1
Coordinates 71827..72114 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag RG44_RS00405 Protein ID WP_000534858.1
Coordinates 72114..72353 (-) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RG44_RS00355 67182..67913 - 732 WP_073544559.1 helix-turn-helix domain-containing protein -
RG44_RS00365 68320..69279 - 960 WP_000592549.1 DUF523 and DUF1722 domain-containing protein -
RG44_RS00370 69472..69996 + 525 WP_000780584.1 lipocalin family protein -
RG44_RS00375 70152..70529 - 378 WP_001204787.1 antitermination protein -
RG44_RS00380 70547..70753 - 207 Protein_64 DUF968 domain-containing protein -
RG44_RS00385 70787..71065 - 279 WP_001515373.1 hypothetical protein -
RG44_RS00390 71132..71383 - 252 WP_001463433.1 hypothetical protein -
RG44_RS00395 71600..71755 - 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
RG44_RS00400 71827..72114 - 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
RG44_RS00405 72114..72353 - 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
RG44_RS00410 72378..72683 + 306 WP_071593935.1 hypothetical protein -
RG44_RS00420 72886..73218 + 333 WP_001301033.1 protein FlxA -
RG44_RS00425 73655..74995 - 1341 WP_000589013.1 ISNCY family transposase -
RG44_RS00440 75764..76050 - 287 Protein_73 hypothetical protein -
RG44_RS27165 76216..76398 - 183 Protein_74 hypothetical protein -
RG44_RS00450 76661..77017 - 357 WP_001315552.1 class I SAM-dependent methyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 64890..103458 38568


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T50948 WP_000323025.1 NZ_CP010160:c72114-71827 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T50948 NZ_CP010160:c72114-71827 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGATCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT50948 WP_000534858.1 NZ_CP010160:c72353-72114 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT50948 NZ_CP010160:c72353-72114 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References