Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-YafN
Location 117085..117620 Replicon plasmid A
Accession NZ_CP010158
Organism Escherichia coli strain D10

Toxin (Protein)


Gene name relE Uniprot ID Q3YTD6
Locus tag RG43_RS27840 Protein ID WP_000222766.1
Coordinates 117085..117372 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID V0VIP2
Locus tag RG43_RS27845 Protein ID WP_001132900.1
Coordinates 117369..117620 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RG43_RS29345 113403..113597 + 195 Protein_112 IS91 family transposase -
RG43_RS29350 113565..113780 - 216 Protein_113 helix-turn-helix transcriptional regulator -
RG43_RS27805 113876..114133 + 258 Protein_114 IS3 family transposase -
RG43_RS27810 114202..115353 + 1152 WP_001254932.1 IS30-like element IS30 family transposase -
RG43_RS28700 115366..115539 - 174 Protein_116 transposase -
RG43_RS28705 115596..116808 + 1213 Protein_117 IS3 family transposase -
RG43_RS27840 117085..117372 - 288 WP_000222766.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
RG43_RS27845 117369..117620 - 252 WP_001132900.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
RG43_RS27860 118584..119441 - 858 WP_000130940.1 incFII family plasmid replication initiator RepA -
RG43_RS27865 119434..119508 - 75 WP_001365705.1 RepA leader peptide Tap -
RG43_RS27875 119734..119994 - 261 WP_073507066.1 replication regulatory protein RepA -
RG43_RS27880 120233..120806 - 574 Protein_123 DUF2726 domain-containing protein -
RG43_RS27885 120844..121053 - 210 WP_001333231.1 hemolysin expression modulator Hha -
RG43_RS27890 121099..121560 - 462 WP_001233871.1 thermonuclease family protein -
RG43_RS27895 121803..122015 - 213 WP_012311405.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sitABCD iutA / iucD / iucC / iucB / iucA / iroB / iroC / iroC / iroD / iroE / iroN / vat 1..128612 128612


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-83)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11147.23 Da        Isoelectric Point: 10.5832

>T50946 WP_000222766.1 NZ_CP010158:c117372-117085 [Escherichia coli]
MTYTVKFRDDALKEWLKLDKTIQQQFVKKLKKCSENPHIPSAKLRGLKDCYKIKLRASGFRLVYQVIDDMLIIAVVAVGK
RERSNVYNLASERMR

Download         Length: 288 bp

>T50946 NZ_CP010158:c117372-117085 [Escherichia coli]
ATGACTTATACGGTAAAATTCAGGGATGATGCGCTCAAGGAATGGCTGAAACTGGATAAGACCATTCAACAACAGTTTGT
GAAAAAACTAAAAAAATGCAGCGAAAATCCTCATATACCGTCAGCAAAACTAAGAGGGCTAAAGGATTGCTACAAAATTA
AACTACGTGCATCGGGATTTCGTCTGGTTTATCAGGTGATTGACGATATGTTAATTATTGCGGTTGTTGCGGTAGGAAAA
CGAGAACGTAGTAACGTTTATAATCTCGCCAGTGAGAGAATGAGATAG

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9211.41 Da        Isoelectric Point: 4.0780

>AT50946 WP_001132900.1 NZ_CP010158:c117620-117369 [Escherichia coli]
MPNIILSDTSASVSELKKNPMATVSAGDGYPVAILNRNQPAFYCVPAELYERMLDALDDQELVKLVTERSNQPLHDVDLD
SYL

Download         Length: 252 bp

>AT50946 NZ_CP010158:c117620-117369 [Escherichia coli]
ATGCCTAACATCATTCTGTCTGACACCAGTGCAAGTGTCAGTGAACTGAAAAAAAATCCTATGGCTACAGTCAGTGCCGG
AGATGGTTACCCTGTCGCCATTCTTAACCGGAATCAGCCGGCATTCTACTGTGTGCCTGCTGAACTGTATGAAAGGATGC
TTGATGCTCTGGATGACCAGGAACTGGTGAAACTGGTTACTGAACGCAGCAATCAGCCACTGCATGATGTGGACCTAGAC
AGTTATTTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CDZ2

Antitoxin

Source ID Structure
AlphaFold DB V0VIP2

References