Detailed information of TA system TA050937

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4913372..4914207
• Replicon: chromosome
• Accession: NZ_CP010157
• Organism: Escherichia coli strain D10

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: F4TIK8
• Locus tag: RG43_RS25900
• Protein ID: WP_000854694.1
• Coordinates: 4913372..4913749 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A8S7S1S5
• Locus tag: RG43_RS25905
• Protein ID: WP_065793167.1
• Coordinates: 4913839..4914207 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RG43_RS25860	4908506..4908799	-	294	WP_001297978.1	hypothetical protein	-
RG43_RS25870	4909021..4909839	+	819	WP_000779405.1	lipoprotein NlpA	-
RG43_RS25875	4909843..4910766	-	924	WP_000535959.1	carboxylate/amino acid/amine transporter	-
RG43_RS25880	4910933..4911430	-	498	WP_000509808.1	hypothetical protein	-
RG43_RS25890	4912026..4912868	-	843	WP_001696593.1	DUF4942 domain-containing protein	-
RG43_RS29290	4912953..4913150	-	198	WP_086795284.1	DUF957 domain-containing protein	-
RG43_RS29295	4913226..4913375	-	150	Protein_4615	hypothetical protein	-
RG43_RS25900	4913372..4913749	-	378	WP_000854694.1	TA system toxin CbtA family protein	Toxin
RG43_RS25905	4913839..4914207	-	369	WP_065793167.1	type IV toxin-antitoxin system toxin CbtA	Antitoxin
RG43_RS25915	4914370..4914591	-	222	WP_000692303.1	DUF987 domain-containing protein	-
RG43_RS25920	4914654..4915130	-	477	WP_001186710.1	RadC family protein	-
RG43_RS25925	4915142..4915621	-	480	WP_000860065.1	antirestriction protein	-
RG43_RS25930	4915703..4916521	-	819	WP_001175149.1	DUF945 domain-containing protein	-
RG43_RS25935	4916611..4916844	-	234	WP_073506913.1	DUF905 family protein	-
RG43_RS25940	4916850..4917527	-	678	WP_073506914.1	hypothetical protein	-
RG43_RS25945	4917646..4918530	-	885	WP_000010392.1	50S ribosome-binding GTPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14008.02 Da
• Isoelectric Point: 7.7293

>T50937 WP_000854694.1 NZ_CP010157:c4913749-4913372 [Escherichia coli]
MKTLPDTHVREASCCPSPVTIWQTLLTRLLDQHYGLALNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPGAKQ

Nucleotide

• Length: 378 bp

>T50937 NZ_CP010157:c4913749-4913372 [Escherichia coli]
ATGAAAACATTACCCGACACGCATGTACGGGAGGCATCGTGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGGTTACTGGACCAGCACTACGGCCTCGCGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AAGCGGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGTTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCACGCCGGGCAACCGGCCTGATGACACGCGA
CAACTACAGAACGGTAAATAACATTACCCTGGGTAAACATCCGGGGGCGAAACAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13555.50 Da
• Isoelectric Point: 6.6249

>AT50937 WP_065793167.1 NZ_CP010157:c4914207-4913839 [Escherichia coli]
VSDALSGTMLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLGSCGYVYMAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT50937 NZ_CP010157:c4914207-4913839 [Escherichia coli]
GTGTCAGACGCACTCTCCGGGACAATGCTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGTCTGACCTGCAAAGCCGACACCCTCGGCAGTTG
TGGCTACGTTTATATGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A1M2F934