Detailed information of TA system TA050928

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2563899..2564535
• Replicon: chromosome
• Accession: NZ_CP010157
• Organism: Escherichia coli strain D10

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A5F1EUB3
• Locus tag: RG43_RS13405
• Protein ID: WP_000813796.1
• Coordinates: 2564359..2564535 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: RG43_RS13400
• Protein ID: WP_076838470.1
• Coordinates: 2563899..2564315 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RG43_RS13380	2559051..2559992	-	942	WP_001251307.1	ABC transporter permease	-
RG43_RS13385	2559993..2561006	-	1014	WP_000220433.1	ABC transporter ATP-binding protein	-
RG43_RS13390	2561024..2562169	-	1146	WP_000047448.1	ABC transporter substrate-binding protein	-
RG43_RS13395	2562414..2563820	-	1407	WP_000760605.1	PLP-dependent aminotransferase family protein	-
RG43_RS13400	2563899..2564315	-	417	WP_076838470.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
RG43_RS13405	2564359..2564535	-	177	WP_000813796.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
RG43_RS13410	2564757..2564987	+	231	WP_000494243.1	YncJ family protein	-
RG43_RS13415	2565079..2567040	-	1962	WP_001317809.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
RG43_RS13420	2567113..2567649	-	537	WP_000429147.1	DNA-binding transcriptional regulator SutR	-
RG43_RS13425	2567702..2568913	+	1212	WP_071593090.1	BenE family transporter YdcO	-
RG43_RS13435	2569022..2569480	-	459	WP_000526135.1	IS200/IS605 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2569022..2569480	458

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6751.78 Da
• Isoelectric Point: 11.5666

>T50928 WP_000813796.1 NZ_CP010157:c2564535-2564359 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFQGRRSVMPRHPSDEIKEPLRKAILKQLGLN

Nucleotide

• Length: 177 bp

>T50928 NZ_CP010157:c2564535-2564359 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTGGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCAAGGGCGACGCAGTGTCATGCCGCGTCACCCCAGTGATGAGATAAAAGAACCATTGCGTAAAGCAATCCTGAAGC
AACTCGGTTTAAATTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15219.59 Da
• Isoelectric Point: 4.5908

>AT50928 WP_076838470.1 NZ_CP010157:c2564315-2563899 [Escherichia coli]
MRYPVTLTPAPEGGYVVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLTNNDHFIEVPLSIASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT50928 NZ_CP010157:c2564315-2563899 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACTCCTGCGCCGGAAGGCGGTTATGTGGTTTCTTTTGTAGATATCCCTGAAGCGTTGAC
GCAGGGAGAAACTGTTGCTGAAGCGATGGAAGCAGCAAAAGATGCCTTACTCACCGCATTTGATTTTTATTTTGAAGATA
ATGAGCTAATCCCGTTGCCTTCGCCATTAACTAACAACGATCACTTTATTGAAGTGCCTTTAAGTATCGCCTCTAAAGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGACGAATTGGCAAACCTAAACAGGAGAT
TACTCGCTTATTTAACTTGCACCATGCTACAAAAATCGACGCCGTCCAGCTTGCGGCTAAGGCGCTTGGCAAAGAGTTAT
CACTGGTGATGGTTTGA

Structures

Toxin

• AlphaFold DB: A0A5F1EUB3