Detailed information of TA system TA050912

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 285868..286499
• Replicon: chromosome
• Accession: NZ_CP010157
• Organism: Escherichia coli strain D10

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: L4J012
• Locus tag: RG43_RS01475
• Protein ID: WP_001260301.1
• Coordinates: 285868..286143 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: L4IZA1
• Locus tag: RG43_RS01480
• Protein ID: WP_000593557.1
• Coordinates: 286140..286499 (+)
• Length: 120 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RG43_RS01460	280872..281939	+	1068	WP_000361470.1	HlyD family secretion protein	-
RG43_RS01465	281936..284671	+	2736	WP_073506899.1	ribosome-associated ATPase/putative transporter RbbA	-
RG43_RS01470	284671..285795	+	1125	WP_001318088.1	ABC transporter permease	-
RG43_RS01475	285868..286143	+	276	WP_001260301.1	type II toxin-antitoxin system HicA family toxin	Toxin
RG43_RS01480	286140..286499	+	360	WP_000593557.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
RG43_RS01485	286619..287020	-	402	WP_001190062.1	nickel-responsive transcriptional regulator NikR	-
RG43_RS01490	287026..287832	-	807	WP_000173650.1	nickel import ATP-binding protein NikE	-
RG43_RS01495	287829..288593	-	765	WP_001136206.1	nickel import ATP-binding protein NikD	-
RG43_RS01500	288593..289426	-	834	WP_001008963.1	nickel ABC transporter permease subunit NikC	-
RG43_RS01505	289423..290367	-	945	WP_000947063.1	nickel ABC transporter permease subunit NikB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (30-80)

Antitoxin

• Uncharacterised protein family HicB (60-112)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10185.87 Da
• Isoelectric Point: 10.6128

>T50912 WP_001260301.1 NZ_CP010157:285868-286143 [Escherichia coli]
MRTQVQALRKKQKNTLDQIFKSPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Nucleotide

• Length: 276 bp

>T50912 NZ_CP010157:285868-286143 [Escherichia coli]
ATGAGAACCCAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAATCTCCTGTTCCTCAAGG
GATTAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTT
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin

Protein

• Length: 120 a.a.
• Molecular weight: 13389.28 Da
• Isoelectric Point: 5.1987

>AT50912 WP_000593557.1 NZ_CP010157:286140-286499 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLTNAAAQQQVSVNTYIIETLNERLNHL

Nucleotide

• Length: 360 bp

>AT50912 NZ_CP010157:286140-286499 [Escherichia coli]
ATGATCAAACTGAAGACACCAAACTCTATGGAGATTGCCGGGCAACCTGCAGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGTATTCAGGGGCTGCAAAAAGAAGGGG
AGCTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACATTACGCTATCCAGAATCGCTGAGTGAACGCCTTACTAATGCGGCTGCGCAGCAACAAGTCTCTGTAAATACTTA
TATTATTGAAACGCTAAATGAGCGTTTGAATCATTTATAA

Structures

Toxin

• AlphaFold DB: L4J012

Antitoxin

• AlphaFold DB: L4IZA1