Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-DnaT
Location 15435..15957 Replicon plasmid C
Accession NZ_CP010155
Organism Escherichia coli strain D9

Toxin (Protein)


Gene name relE Uniprot ID G3CAN5
Locus tag RG42_RS25525 Protein ID WP_000220560.1
Coordinates 15676..15957 (+) Length 94 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID G3CAG1
Locus tag RG42_RS25520 Protein ID WP_000121743.1
Coordinates 15435..15686 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RG42_RS25485 10798..11028 + 231 WP_000051064.1 partitioning protein -
RG42_RS25490 11044..11337 + 294 WP_015060510.1 hypothetical protein -
RG42_RS25495 11339..12307 + 969 WP_077249520.1 IS5-like element IS903B family transposase -
RG42_RS25500 12367..12789 - 423 WP_031942371.1 DNA distortion polypeptide 3 -
RG42_RS25505 12857..13303 - 447 WP_001074386.1 hypothetical protein -
RG42_RS25510 13343..14179 - 837 WP_001050931.1 replication initiation protein -
RG42_RS25520 15435..15686 + 252 WP_000121743.1 plasmid stabilization protein Antitoxin
RG42_RS25525 15676..15957 + 282 WP_000220560.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
RG42_RS25530 16103..16426 + 324 WP_001181903.1 hypothetical protein -
RG42_RS25535 16471..16716 + 246 WP_000356546.1 hypothetical protein -
RG42_RS25540 16706..16921 + 216 WP_001180116.1 hypothetical protein -
RG42_RS25545 17014..17343 + 330 WP_000866648.1 hypothetical protein -
RG42_RS26820 17630..17779 + 150 WP_000003880.1 hypothetical protein -
RG42_RS25550 17792..18064 + 273 WP_000160399.1 hypothetical protein -
RG42_RS25555 18391..18936 + 546 WP_038976855.1 hypothetical protein -
RG42_RS25560 18939..20096 + 1158 WP_000538023.1 hypothetical protein -
RG42_RS25565 20457..20948 + 492 WP_000872475.1 transcription termination factor NusG -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid floR / aph(3')-Ia / qnrS2 - 1..39406 39406


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 11005.83 Da        Isoelectric Point: 10.5938

>T50908 WP_000220560.1 NZ_CP010155:15676-15957 [Escherichia coli]
MTYELAFDRRALKEWQKLGHTIREQFKKKLAERLENPRVPAARLHGHADRYKIKLRASGYRLVYQVIDEKVVLLVIAVGR
RESSEVYQIADLR

Download         Length: 282 bp

>T50908 NZ_CP010155:15676-15957 [Escherichia coli]
ATGACCTATGAACTGGCTTTTGACCGTAGAGCACTGAAGGAATGGCAGAAACTCGGCCACACCATCCGTGAACAATTCAA
AAAGAAACTGGCAGAACGGCTGGAAAATCCACGCGTACCCGCAGCCCGGTTACATGGTCATGCTGATCGCTATAAAATCA
AACTTCGTGCATCTGGCTACAGACTTGTATACCAAGTCATTGATGAGAAAGTCGTTTTACTCGTTATTGCCGTTGGAAGA
AGGGAAAGCAGCGAAGTCTATCAGATCGCAGATTTGCGCTAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9194.43 Da        Isoelectric Point: 4.1683

>AT50908 WP_000121743.1 NZ_CP010155:15435-15686 [Escherichia coli]
MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCVPPKLYAYYRELAEDAELNAVADERMKNPEIVKVNL
DDL

Download         Length: 252 bp

>AT50908 NZ_CP010155:15435-15686 [Escherichia coli]
ATGAGCTATCAAATTCTGACAACCACAGCGGCCAGTATTACTGACCTGAAAAAAAATCCTATGGGAACCGTAGCTGAAGG
TGAAGGGGACGCTGTTGCGATCCTGAACCGAAACGAACCGGCGTTCTATTGCGTTCCACCAAAACTTTACGCCTACTATC
GGGAACTCGCTGAAGATGCTGAGTTAAACGCTGTTGCTGATGAGCGCATGAAAAACCCGGAAATTGTGAAGGTTAACCTG
GATGACCTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB G3CAN5

Antitoxin

Source ID Structure
AlphaFold DB A0A3S5I301

References