Detailed information of TA system TA050906

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-RelB
• Location: 102345..102943
• Replicon: plasmid A
• Accession: NZ_CP010153
• Organism: Escherichia coli strain D9

Toxin (Protein)

• Gene name: doc
• Uniprot ID: A0A8T6PRV3
• Locus tag: RG42_RS24875
• Protein ID: WP_053291281.1
• Coordinates: 102566..102943 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: L4J1C0
• Locus tag: RG42_RS24870
• Protein ID: WP_001603498.1
• Coordinates: 102345..102566 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RG42_RS24855	97945..98625	+	681	WP_032187672.1	fimbrial assembly chaperone	-
RG42_RS24860	98643..101129	+	2487	WP_073503570.1	fimbria/pilus outer membrane usher protein	-
RG42_RS24865	101141..102160	+	1020	WP_073503571.1	type 1 fimbrial protein	-
RG42_RS24870	102345..102566	+	222	WP_001603498.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
RG42_RS24875	102566..102943	+	378	WP_053291281.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
RG42_RS24880	103077..104192	-	1116	WP_032187668.1	DNA primase	-
RG42_RS24885	104349..105689	-	1341	WP_073503572.1	AAA family ATPase	-
RG42_RS24890	105750..106475	-	726	WP_073503573.1	hypothetical protein	-
RG42_RS24895	106758..107525	+	768	WP_000342417.1	hypothetical protein	-
RG42_RS24900	107578..107931	-	354	WP_160378290.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..114159	114159

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Fido domain (4-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-51)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13487.28 Da
• Isoelectric Point: 6.2257

>T50906 WP_053291281.1 NZ_CP010153:102566-102943 [Escherichia coli]
MRHISPEELIAIHDANISRYGGLPGMPDSGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVYDSPELAELTVGAATGEVSVSTVVATLRRLYGTA

Nucleotide

• Length: 378 bp

>T50906 NZ_CP010153:102566-102943 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAGCTTATTGCAATTCATGATGCGAATATAAGCCGCTACGGCGGCCTGCCAGGCATGCC
AGATTCGGGAAGAGCTGAGGCTATTATCGGGAGAGTCCAGGCCAGAGTTGCATATGAAGAGATTACCGACCTTTTCGAAG
TCTCCGCAACTTACCTGGTGGCTACTGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
TTGTTATTTCTGCGCCGTAACGGTGTGCAGGTATATGATTCACCAGAACTGGCAGAGCTTACTGTGGGGGCTGCGACCGG
AGAGGTATCCGTATCTACTGTCGTTGCCACGTTACGTAGATTGTATGGTACGGCGTAG

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8190.24 Da
• Isoelectric Point: 6.3159

>AT50906 WP_001603498.1 NZ_CP010153:102345-102566 [Escherichia coli]
MQSINFRTARSNLTEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDTTNKKLVNR

Nucleotide

• Length: 222 bp

>AT50906 NZ_CP010153:102345-102566 [Escherichia coli]
ATGCAATCTATTAACTTCCGTACCGCACGCAGCAATCTTACCGAAGTGCTCAACAATGTTGAAGCCGGAGAAGAGGTTGA
AATCACCCGTAGAGGCCGTGAGCCAGCTGTAATTGTCAGCAAGGCCACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTCGCATCCCTGTTTGACACTCTGGACACCACCAATAAGAAACTGGTTAACCGATAA

Structures

Antitoxin

• AlphaFold DB: L4J1C0