Detailed information of TA system TA050879

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 5069433..5070231
• Replicon: chromosome
• Accession: NZ_CP010151
• Organism: Escherichia coli strain D8

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A1M0HL60
• Locus tag: RG41_RS26355
• Protein ID: WP_000854692.1
• Coordinates: 5069433..5069810 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A1L4NTN5
• Locus tag: RG41_RS26360
• Protein ID: WP_001605875.1
• Coordinates: 5069857..5070231 (-)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RG41_RS26330	5064572..5065720	-	1149	WP_000905920.1	capsule polysaccharide export inner-membrane protein KpsE	-
RG41_RS26335	5065792..5066775	-	984	WP_074434986.1	KpsF/GutQ family sugar-phosphate isomerase	-
RG41_RS26340	5067574..5067744	-	171	Protein_4718	IS110 family transposase	-
RG41_RS26345	5068087..5068929	-	843	WP_001280485.1	DUF4942 domain-containing protein	-
RG41_RS27885	5069014..5069211	-	198	WP_023563519.1	DUF957 domain-containing protein	-
RG41_RS27890	5069287..5069436	-	150	Protein_4721	hypothetical protein	-
RG41_RS26355	5069433..5069810	-	378	WP_000854692.1	TA system toxin CbtA family protein	Toxin
RG41_RS26360	5069857..5070231	-	375	WP_001605875.1	type IV toxin-antitoxin system toxin CbtA	Antitoxin
RG41_RS26365	5070307..5070528	-	222	WP_000692323.1	DUF987 domain-containing protein	-
RG41_RS26370	5070597..5071073	-	477	WP_001186181.1	RadC family protein	-
RG41_RS26375	5071089..5071574	-	486	WP_000213727.1	antirestriction protein	-
RG41_RS26380	5071666..5072484	-	819	WP_001234393.1	DUF945 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	5067574..5067678	104

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14119.12 Da
• Isoelectric Point: 7.3249

>T50879 WP_000854692.1 NZ_CP010151:c5069810-5069433 [Escherichia coli]
MKTLPDTHVREASCCPSPVTIWHTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEAKQ

Nucleotide

• Length: 378 bp

>T50879 NZ_CP010151:c5069810-5069433 [Escherichia coli]
ATGAAAACATTACCTGACACACACGTACGGGAGGCATCGTGCTGCCCGTCTCCCGTCACCATCTGGCATACACTGCTCAC
CCGACTGCTGGACCAGCATTACGGCCTCACACTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACCGAACAGTAAATAACATTACTCTGGGTAAGCATCCGGAGGCGAAACAATGA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 13594.42 Da
• Isoelectric Point: 5.4554

>AT50879 WP_001605875.1 NZ_CP010151:c5070231-5069857 [Escherichia coli]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLACEADTLGSCGYVYMAVYPTLAPATTS

Nucleotide

• Length: 375 bp

>AT50879 NZ_CP010151:c5070231-5069857 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAGCTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGGCCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATATGGCTGTTTATCCGACACTGGCACCCGCAACCACCTCATAA

Structures

Toxin

• AlphaFold DB: A0A1M0HL60

Antitoxin

• AlphaFold DB: A0A1L4NTN5