Detailed information of TA system TA050875

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3698453..3699251
• Replicon: chromosome
• Accession: NZ_CP010151
• Organism: Escherichia coli strain D8

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: RG41_RS19300
• Protein ID: WP_001609485.1
• Coordinates: 3698874..3699251 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: RG41_RS19295
• Protein ID: WP_001609483.1
• Coordinates: 3698453..3698827 (+)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RG41_RS19250	3693611..3694291	+	681	WP_001608660.1	WYL domain-containing protein	-
RG41_RS19255	3694552..3694830	+	279	WP_001545361.1	hypothetical protein	-
RG41_RS19265	3695169..3695480	+	312	WP_001608658.1	hypothetical protein	-
RG41_RS19270	3695579..3696397	+	819	WP_001609564.1	DUF945 domain-containing protein	-
RG41_RS19275	3696479..3696958	+	480	WP_001609479.1	antirestriction protein	-
RG41_RS19280	3696974..3697450	+	477	WP_001186188.1	RadC family protein	-
RG41_RS19285	3697519..3697740	+	222	WP_001609480.1	DUF987 domain-containing protein	-
RG41_RS19290	3697759..3698403	+	645	WP_001609482.1	hypothetical protein	-
RG41_RS19295	3698453..3698827	+	375	WP_001609483.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
RG41_RS19300	3698874..3699251	+	378	WP_001609485.1	TA system toxin CbtA family protein	Toxin
RG41_RS19305	3699248..3699736	+	489	WP_001609486.1	hypothetical protein	-
RG41_RS19310	3699748..3699945	+	198	WP_001609487.1	DUF957 domain-containing protein	-
RG41_RS19315	3700041..3700885	+	845	Protein_3460	DUF4942 domain-containing protein	-
RG41_RS19320	3700956..3702491	+	1536	WP_001609560.1	EAL domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14276.23 Da
• Isoelectric Point: 7.2141

>T50875 WP_001609485.1 NZ_CP010151:3698874-3699251 [Escherichia coli]
MKTLPDTHVREASRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPEF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITQGKYPEAKQ

Nucleotide

• Length: 378 bp

>T50875 NZ_CP010151:3698874-3699251 [Escherichia coli]
ATGAAAACATTACCTGACACACACGTACGGGAGGCATCGCGTTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTACGGCCTCACGCTGAATGACACTCCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCTGGCATTTCACTGTGCGATGCAGTGAACTTTCTTGTTGAAAAATACGCACTGGTGCGTACCGACCAGCCGGAATTC
AGTGCCTGTACCCGTTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCAGGGTAAGTATCCGGAGGCGAAACAATGA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 13675.54 Da
• Isoelectric Point: 6.4603

>AT50875 WP_001609483.1 NZ_CP010151:3698453-3698827 [Escherichia coli]
VSDTLPGTTLPDDNNDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYHLDQAFPLLMKQLKLMLTSG
ELNPCHQHTVTLYAKGLTCEADTFGSCGYVYLAVYPTLAPATTS

Nucleotide

• Length: 375 bp

>AT50875 NZ_CP010151:3698453-3698827 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACGCTTCCCGACGACAATAACGACCGCCCCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTTGGCGCCCGTCTGGTGCAGGAAGGGAACCGGTTGCATTACCTTGCAGACCGCGCCGGTATCAGAGGCCGGT
TCAGGGACGCAGATGCATACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGAAATTAATGCTCACCAGCGGT
GAACTGAATCCCTGCCATCAGCATACCGTCACACTGTATGCGAAAGGGCTGACCTGCGAAGCCGACACCTTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACTGGCACCCGCAACCACTTCATAA