Detailed information of TA system TA050874

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yjhX-yjhQ/YjhX-GNAT
• Location: 3301470..3302290
• Replicon: chromosome
• Accession: NZ_CP010151
• Organism: Escherichia coli strain D8

Toxin (Protein)

• Gene name: yjhX
• Uniprot ID: S1PA82
• Locus tag: RG41_RS17095
• Protein ID: WP_001054376.1
• Coordinates: 3301470..3301727 (+)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: yjhQ
• Uniprot ID: -
• Locus tag: RG41_RS17100
• Protein ID: WP_001519303.1
• Coordinates: 3301739..3302290 (+)
• Length: 184 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RG41_RS17070	3296756..3297862	+	1107	WP_001519308.1	N-acetylneuraminate epimerase	-
RG41_RS17075	3297927..3298907	+	981	WP_001519307.1	transposase	-
RG41_RS17085	3299490..3300731	-	1242	Protein_3080	helicase YjhR	-
RG41_RS17090	3300794..3301093	+	300	WP_001519304.1	GNAT family N-acetyltransferase	-
RG41_RS17095	3301470..3301727	+	258	WP_001054376.1	hypothetical protein	Toxin
RG41_RS17100	3301739..3302290	+	552	WP_001519303.1	N-acetyltransferase	Antitoxin
RG41_RS28040	3302342..3302503	+	162	WP_001519302.1	hypothetical protein	-
RG41_RS17105	3302540..3303088	+	549	WP_001519301.1	hypothetical protein	-
RG41_RS17110	3303215..3303475	+	261	WP_000084876.1	hypothetical protein	-
RG41_RS28215	3303513..3303629	+	117	Protein_3087	VOC family protein	-
RG41_RS17120	3303874..3304995	+	1122	WP_000010833.1	M42 family metallopeptidase	-
RG41_RS17125	3304992..3305270	+	279	WP_000722973.1	PTS sugar transporter subunit IIB	-
RG41_RS17130	3305282..3306595	+	1314	WP_001519300.1	galactitol-specific PTS transporter subunit IIC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fimE / fimB	3292880..3310510	17630

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Putative toxin of bacterial toxin-antitoxin pair (1-85)

Antitoxin

• GNAT domain (18-121)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 9734.29 Da
• Isoelectric Point: 11.0090

>T50874 WP_001054376.1 NZ_CP010151:3301470-3301727 [Escherichia coli]
MNLSRQEQHTLHVLAKGRRIAHVRDSSGRVTSVECYSREGLLLTDCTLAVFKKLKTKKLIKSVNGQPYRINTTELNKVRA
QLDNR

Nucleotide

• Length: 258 bp

>T50874 NZ_CP010151:3301470-3301727 [Escherichia coli]
ATGAATTTATCCCGTCAGGAACAACATACCTTACACGTTCTCGCTAAAGGTAGACGTATTGCGCACGTCCGCGATTCTTC
AGGCCGCGTCACTTCCGTTGAATGCTACAGCCGCGAAGGGCTGTTGCTGACCGACTGCACGCTCGCCGTCTTCAAAAAAC
TCAAAACCAAAAAACTTATCAAGTCCGTCAATGGCCAGCCCTATCGAATCAACACCACCGAGCTGAATAAAGTTCGCGCT
CAGCTCGATAATCGCTAA

Antitoxin

Protein

• Length: 184 a.a.
• Molecular weight: 20463.61 Da
• Isoelectric Point: 6.8758

>AT50874 WP_001519303.1 NZ_CP010151:3301739-3302290 [Escherichia coli]
MTVHHFTFHITDKSDASDIREVETRAFGFSKEADLVASLLEDESARPALSLLARYEGKAVGHILFTRATFKGEMDSPLMH
ILAPLAVIPEYQGMGVGGRLIRTGIEHLRLMGCQTVFVLGHATYYPRHGFEPCAGDKGYPAPYPIPEEHKACWMMQSLTA
QPMTLTGHIRCAQALMKPEHWRE

Nucleotide

• Length: 552 bp

>AT50874 NZ_CP010151:3301739-3302290 [Escherichia coli]
ATGACAGTACACCACTTCACTTTTCATATCACAGATAAAAGTGATGCCAGTGATATCCGGGAAGTCGAAACCCGCGCATT
TGGCTTCAGCAAAGAAGCCGATTTAGTGGCCTCTTTACTTGAGGATGAAAGCGCGCGCCCCGCGTTATCGCTGCTAGCCC
GGTATGAAGGCAAAGCCGTCGGGCATATTTTATTTACCCGGGCGACCTTCAAAGGAGAAATGGATTCACCGCTGATGCAC
ATCCTCGCTCCCCTGGCGGTCATACCGGAATACCAAGGGATGGGCGTGGGAGGACGGTTGATACGCACTGGCATCGAGCA
CCTGAGGTTGATGGGCTGCCAGACGGTTTTTGTTCTCGGCCACGCCACCTATTACCCCCGACATGGGTTCGAACCCTGTG
CCGGAGACAAAGGCTATCCGGCACCTTATCCCATCCCGGAGGAGCACAAAGCCTGCTGGATGATGCAATCACTCACCGCT
CAGCCCATGACCCTGACCGGCCACATCCGGTGCGCGCAGGCCCTGATGAAACCGGAGCACTGGCGTGAATAA

Structures

Toxin

• AlphaFold DB: S1PA82