50867

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 2701458..2702076 Replicon chromosome
Accession NZ_CP010151
Organism Escherichia coli strain D8

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag RG41_RS14040 Protein ID WP_001291435.1
Coordinates 2701858..2702076 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag RG41_RS14035 Protein ID WP_000344800.1
Coordinates 2701458..2701832 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RG41_RS14025 2696548..2697741 + 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
RG41_RS14030 2697764..2700913 + 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
RG41_RS14035 2701458..2701832 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
RG41_RS14040 2701858..2702076 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
RG41_RS14045 2702249..2702800 + 552 WP_000102539.1 maltose O-acetyltransferase -
RG41_RS14050 2702916..2703386 + 471 WP_000136192.1 YlaC family protein -
RG41_RS14055 2703550..2705100 + 1551 WP_001468081.1 cyclic-guanylate-specific phosphodiesterase PdeB -
RG41_RS14060 2705142..2705495 - 354 WP_000878142.1 DUF1428 family protein -
RG41_RS14070 2705874..2706185 + 312 WP_000409908.1 MGMT family protein -
RG41_RS14075 2706216..2706788 - 573 WP_000779844.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T50867 WP_001291435.1 NZ_CP010151:2701858-2702076 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T50867 NZ_CP010151:2701858-2702076 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT50867 WP_000344800.1 NZ_CP010151:2701458-2701832 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT50867 NZ_CP010151:2701458-2701832 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References