Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3215775..3216429 Replicon chromosome
Accession NZ_CP010150
Organism Escherichia coli strain D7

Toxin (Protein)


Gene name cptA Uniprot ID F4NJ21
Locus tag RG40_RS16815 Protein ID WP_000244772.1
Coordinates 3216022..3216429 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag RG40_RS16810 Protein ID WP_000354046.1
Coordinates 3215775..3216041 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RG40_RS16785 3211063..3211806 + 744 WP_023155906.1 SDR family oxidoreductase -
RG40_RS16790 3211863..3213296 - 1434 WP_001363803.1 6-phospho-beta-glucosidase BglA -
RG40_RS16795 3213341..3213652 + 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
RG40_RS16800 3213816..3214475 + 660 WP_000250274.1 hemolysin III family protein -
RG40_RS16805 3214552..3215532 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
RG40_RS16810 3215775..3216041 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
RG40_RS16815 3216022..3216429 + 408 WP_000244772.1 protein YgfX Toxin
RG40_RS16820 3216469..3216990 - 522 WP_001055874.1 flavodoxin FldB -
RG40_RS16825 3217102..3217998 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
RG40_RS16830 3218023..3218733 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
RG40_RS16835 3218739..3220472 + 1734 WP_023155905.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16048.02 Da        Isoelectric Point: 11.2511

>T50839 WP_000244772.1 NZ_CP010150:3216022-3216429 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T50839 NZ_CP010150:3216022-3216429 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGTGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT50839 WP_000354046.1 NZ_CP010150:3215775-3216041 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT50839 NZ_CP010150:3215775-3216041 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYB4

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References