Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 177852..178495 Replicon plasmid A
Accession NZ_CP010149
Organism Escherichia coli strain D6

Toxin (Protein)


Gene name vagD Uniprot ID Q84A06
Locus tag RG39_RS25445 Protein ID WP_000754566.1
Coordinates 178079..178495 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID Q84A07
Locus tag RG39_RS25440 Protein ID WP_001261276.1
Coordinates 177852..178082 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RG39_RS25405 172969..173238 + 270 WP_000339857.1 hypothetical protein -
RG39_RS25410 173415..174281 - 867 WP_004118283.1 replication initiation protein -
RG39_RS26755 174811..174915 + 105 WP_032409716.1 hypothetical protein -
RG39_RS25415 175044..175301 + 258 WP_000764642.1 hypothetical protein -
RG39_RS25420 175359..176135 - 777 WP_000015958.1 site-specific integrase -
RG39_RS25425 176132..176875 - 744 WP_000129823.1 hypothetical protein -
RG39_RS25430 176926..177276 - 351 WP_000493378.1 hypothetical protein -
RG39_RS25440 177852..178082 + 231 WP_001261276.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
RG39_RS25445 178079..178495 + 417 WP_000754566.1 type II toxin-antitoxin system VapC family toxin Toxin
RG39_RS26140 178739..179424 + 686 Protein_201 IS3-like element ISEc15 family transposase -
RG39_RS25470 180097..181116 + 1020 Protein_202 group II intron reverse transcriptase/maturase -
RG39_RS25475 181177..181881 + 705 WP_001067858.1 IS6-like element IS26 family transposase -
RG39_RS25485 181954..182193 + 240 WP_011264039.1 macrolide transporter -
RG39_RS25490 182339..183202 + 864 WP_000612791.1 SDR family oxidoreductase -
RG39_RS25495 183240..183485 + 246 Protein_206 GrpB family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sul2 / aph(3'')-Ib / aph(6)-Id / tet(A) / blaCTX-M-14 / blaTEM-1B / sitABCD / aac(3)-IId / aadA5 / qacE / sul1 / mph(A) / sul3 / ant(3'')-Ia / cmlA1 / aadA2 / dfrA12 iutA / iucD / iucC / iucB / iucA / iroB / iroC / iroD / iroE / iroN 1..199494 199494


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15006.52 Da        Isoelectric Point: 9.2957

>T50830 WP_000754566.1 NZ_CP010149:178079-178495 [Escherichia coli]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAVLPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNVREFARVPGLVLEDWVK

Download         Length: 417 bp

>T50830 NZ_CP010149:178079-178495 [Escherichia coli]
ATGAAGAAAACCTGGATGCTCGATACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACATATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCGTCCTGCCCTGGGACCGCGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTAGCCGGGACGCCGATCGGCCCGAATGACACGGCCATTGC
CGGGCACGCCATCGCGGCGGGCGCCATCCTGGTGACGAACAATGTGAGAGAGTTTGCGCGGGTCCCGGGCCTGGTGCTGG
AAGACTGGGTGAAATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8721.74 Da        Isoelectric Point: 4.3533

>AT50830 WP_001261276.1 NZ_CP010149:177852-178082 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT50830 NZ_CP010149:177852-178082 [Escherichia coli]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGATCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGTTTTGAGCCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A387K1G3

Antitoxin

Source ID Structure
AlphaFold DB A0A387K023

References