Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 1399358..1399934 Replicon chromosome
Accession NZ_CP010148
Organism Escherichia coli strain D6

Toxin (Protein)


Gene name shpA Uniprot ID A0A811CE22
Locus tag RG39_RS07240 Protein ID WP_001402820.1
Coordinates 1399647..1399934 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A1X0YJR8
Locus tag RG39_RS07235 Protein ID WP_001402821.1
Coordinates 1399358..1399660 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RG39_RS07215 1395049..1395561 + 513 WP_001175455.1 4-hydroxyphenylacetate 3-monooxygenase reductase subunit -
RG39_RS07220 1395954..1398104 + 2151 WP_001298933.1 pyruvate/proton symporter BtsT -
RG39_RS07225 1398154..1398357 + 204 WP_000467859.1 YbdD/YjiX family protein -
RG39_RS07230 1398368..1399324 + 957 WP_001295525.1 GTPase -
RG39_RS07235 1399358..1399660 - 303 WP_001402821.1 BrnA antitoxin family protein Antitoxin
RG39_RS07240 1399647..1399934 - 288 WP_001402820.1 BrnT family toxin Toxin
RG39_RS07245 1400131..1401297 + 1167 WP_000800831.1 restriction endonuclease -
RG39_RS07250 1401361..1402980 + 1620 WP_001029742.1 type I restriction-modification system subunit M -
RG39_RS07255 1402970..1404376 + 1407 WP_001402819.1 restriction endonuclease subunit S -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11169.62 Da        Isoelectric Point: 7.4697

>T50803 WP_001402820.1 NZ_CP010148:c1399934-1399647 [Escherichia coli]
MPMEFEWDANKAKSNRVKHGIRFEDAVLVFDDPQHLSQQDRIENGEYRWQTIGVVHGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T50803 NZ_CP010148:c1399934-1399647 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGCAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTGGTGTTTGATGATCCACAACATTTGTCACAGCAAGATCGCATAGAAAACGGTGAGTATCGCTGGCAGACTATCGGCG
TGGTTCACGGTATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGTGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAATCGCTATGAGCATGGTTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11534.04 Da        Isoelectric Point: 9.4646

>AT50803 WP_001402821.1 NZ_CP010148:c1399660-1399358 [Escherichia coli]
MSMVKHKRGTSSILDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT50803 NZ_CP010148:c1399660-1399358 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTATACTCGATGTGCAGCATGAGGCCGAACTGAAGGCGCTGGCGAA
TAAATCCGACGAAGATATTGATTACAGCGATATCCCACCTTCCAGCGATGAGCAATGGTCTAACGCAGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAGGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A811CE22

Antitoxin

Source ID Structure
AlphaFold DB A0A1X0YJR8

References