Detailed information of TA system TA050800

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/-
• Location: 70177..70553
• Replicon: plasmid B
• Accession: NZ_CP010147
• Organism: Escherichia coli strain D5

Toxin (Protein)

• Gene name: parE
• Uniprot ID: A0A075MDK3
• Locus tag: RG38_RS26465
• Protein ID: WP_000969988.1
• Coordinates: 70371..70553 (+)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: -
• Locus tag: RG38_RS26460
• Protein ID: WP_001324224.1
• Coordinates: 70177..70374 (+)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RG38_RS27725	65437..65673	+	237	WP_001323890.1	colicin V immunity protein	-
RG38_RS26440	65651..65962	+	312	WP_001259758.1	colicin V	-
RG38_RS26445	66132..68246	-	2115	WP_001183604.1	peptidase domain-containing ABC transporter	-
RG38_RS26450	68221..69495	-	1275	WP_000489609.1	HlyD family secretion protein	-
RG38_RS26460	70177..70374	+	198	WP_001324224.1	toxin-antitoxin system protein	Antitoxin
RG38_RS26465	70371..70553	+	183	WP_000969988.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
RG38_RS26470	70652..70960	+	309	WP_000377483.1	hypothetical protein	-
RG38_RS26480	71520..72554	+	1035	WP_001190234.1	3-deoxy-7-phosphoheptulonate synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	sitABCD	iucA / iucB / iucC / iucD / iutA / iroN / iroE / iroD / iroC / iroB	1..88754	88754

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• GNAT domain (36-153)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6931.07 Da
• Isoelectric Point: 4.5349

>T50800 WP_000969988.1 NZ_CP010147:70371-70553 [Escherichia coli]
MMEIFWTMLASQDRKRIREYIAEQNLIAAIELDERIGCSASLLFSLSFISVQVHDNIITV

Nucleotide

• Length: 183 bp

>T50800 NZ_CP010147:70371-70553 [Escherichia coli]
ATGATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGAT
AGCCGCCATTGAACTGGACGAACGGATTGGTTGTTCAGCCAGCCTCCTGTTCTCCCTTTCTTTTATCAGTGTACAGGTGC
ATGACAACATAATCACGGTATGA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 7658.53 Da
• Isoelectric Point: 7.2046

>AT50800 WP_001324224.1 NZ_CP010147:70177-70374 [Escherichia coli]
MDRNGSQLIRDFMRQTVERQHNTWFRDQVEAGRQQLERGDVLPHDMVESSAAAWRDEMSRKVAGK

Nucleotide

• Length: 198 bp

>AT50800 NZ_CP010147:70177-70374 [Escherichia coli]
ATGGACCGTAATGGCTCTCAGTTAATCCGGGATTTTATGCGCCAGACCGTGGAACGGCAGCATAATACCTGGTTCCGTGA
CCAGGTTGAGGCAGGACGTCAGCAACTCGAGCGCGGCGATGTGCTTCCCCATGACATGGTCGAATCTTCCGCTGCTGCAT
GGCGGGATGAAATGAGCAGGAAAGTCGCCGGTAAATGA

Structures

Toxin

• AlphaFold DB: A0A075MDK3