Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-YafN
Location 30719..31254 Replicon plasmid A
Accession NZ_CP010146
Organism Escherichia coli strain D5

Toxin (Protein)


Gene name relE Uniprot ID A0A0K4CX11
Locus tag RG38_RS25350 Protein ID WP_000222771.1
Coordinates 30967..31254 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID T1PXV2
Locus tag RG38_RS25345 Protein ID WP_001139206.1
Coordinates 30719..30970 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RG38_RS25305 25887..26576 - 690 WP_050858711.1 EAL domain-containing protein -
RG38_RS25310 26810..27531 + 722 Protein_32 AraC family transcriptional regulator -
RG38_RS25315 27570..27818 - 249 WP_000747846.1 hypothetical protein -
RG38_RS25320 27815..28255 - 441 WP_023352064.1 hypothetical protein -
RG38_RS25325 28289..30106 - 1818 Protein_35 helicase -
RG38_RS25345 30719..30970 + 252 WP_001139206.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
RG38_RS25350 30967..31254 + 288 WP_000222771.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
RG38_RS25355 31544..31744 - 201 WP_000896262.1 DUF839 domain-containing protein -
RG38_RS27130 31734..31939 + 206 Protein_39 transposase -
RG38_RS25360 32114..35611 - 3498 WP_001038839.1 autotransporter outer membrane beta-barrel domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid blaCTX-M-65 / tet(A) / sul1 / floR / sul2 / aph(4)-Ia / aac(3)-IVa / dfrA17 - 1..112874 112874


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-83)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11116.17 Da        Isoelectric Point: 10.5833

>T50791 WP_000222771.1 NZ_CP010146:30967-31254 [Escherichia coli]
MTYTVKFRDDALKEWLRLDKTIQQQFAKKLKKCSENPHIASAKLRGLKDCYKIKLRASGFRLVYQVIDDMLIIAVVAVGK
RERSNVYHLASERMK

Download         Length: 288 bp

>T50791 NZ_CP010146:30967-31254 [Escherichia coli]
ATGACTTATACAGTAAAATTCCGGGATGATGCACTGAAAGAATGGCTCAGACTGGACAAGACCATTCAGCAGCAGTTCGC
AAAAAAACTGAAAAAATGCAGTGAAAATCCTCATATAGCTTCTGCAAAACTACGTGGATTAAAGGACTGCTATAAAATCA
AACTTCGCGCATCAGGTTTTCGTCTGGTTTATCAGGTTATTGATGATATGTTAATTATTGCGGTCGTCGCAGTAGGTAAA
CGTGAACGCAGCAACGTTTATCATCTCGCCAGTGAACGGATGAAATAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9171.39 Da        Isoelectric Point: 4.0927

>AT50791 WP_001139206.1 NZ_CP010146:30719-30970 [Escherichia coli]
MPSIILSDTSASVSELKKNPMATVSAGEGYPVAILNRNQPAFYCVPAELYERMLDALDDQELVKLVTERSSQPLHDVDLD
SYL

Download         Length: 252 bp

>AT50791 NZ_CP010146:30719-30970 [Escherichia coli]
ATGCCCAGTATCATTTTGTCTGACACCAGTGCAAGCGTCAGTGAGCTGAAAAAAAATCCGATGGCAACTGTCAGTGCCGG
AGAGGGGTATCCTGTTGCCATTCTTAACCGGAATCAGCCAGCATTCTACTGTGTCCCTGCTGAGCTCTATGAAAGAATGC
TTGATGCCTTGGATGACCAGGAGCTGGTAAAACTGGTTACTGAACGCAGCAGTCAGCCTCTGCATGATGTGGACCTGGAC
AGTTATTTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0K4CX11

Antitoxin

Source ID Structure
AlphaFold DB T1PXV2

References