Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-Phd
Location 13852..14453 Replicon plasmid A
Accession NZ_CP010146
Organism Escherichia coli strain D5

Toxin (Protein)


Gene name doc Uniprot ID U9YA20
Locus tag RG38_RS25195 Protein ID WP_001216045.1
Coordinates 14073..14453 (+) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID U9YQH9
Locus tag RG38_RS25190 Protein ID WP_001190712.1
Coordinates 13852..14073 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RG38_RS25180 11489..12097 - 609 WP_073511585.1 restriction endonuclease subunit S -
RG38_RS25185 12113..13669 - 1557 WP_016231378.1 type I restriction-modification system subunit M -
RG38_RS25190 13852..14073 + 222 WP_001190712.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
RG38_RS25195 14073..14453 + 381 WP_001216045.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
RG38_RS25200 14458..14637 + 180 WP_000113019.1 hypothetical protein -
RG38_RS25205 14665..15708 + 1044 WP_016231380.1 DUF968 domain-containing protein -
RG38_RS25210 15797..16249 + 453 WP_050858701.1 Late promoter-activating protein -
RG38_RS25215 16336..16888 + 553 Protein_19 hypothetical protein -
RG38_RS25220 16888..18372 + 1485 WP_000124150.1 terminase -
RG38_RS25225 18397..19248 - 852 WP_000611656.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid blaCTX-M-65 / tet(A) / sul1 / floR / sul2 / aph(4)-Ia / aac(3)-IVa / dfrA17 - 1..112874 112874


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(3-120)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-51)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 13588.29 Da        Isoelectric Point: 5.1514

>T50790 WP_001216045.1 NZ_CP010146:14073-14453 [Escherichia coli]
MRHISPEELIALHDANISRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Download         Length: 381 bp

>T50790 NZ_CP010146:14073-14453 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAGCCGCTACGGCGGCCTGCCGGGAATGTC
AGATCCGGGTAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGCGCG
CTGCTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACCGTAGGGGCTGCGACCGG
AGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCTGCGGAGTAG

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8133.10 Da        Isoelectric Point: 4.7708

>AT50790 WP_001190712.1 NZ_CP010146:13852-14073 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Download         Length: 222 bp

>AT50790 NZ_CP010146:13852-14073 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGCGGCAACCTTTCTGAAGTGCTCAACAATGTTGAAGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CLP7

Antitoxin

Source ID Structure
AlphaFold DB A0A829CJB6

References