Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4534750..4535368 Replicon chromosome
Accession NZ_CP010145
Organism Escherichia coli strain D5

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag RG38_RS23580 Protein ID WP_001291435.1
Coordinates 4534750..4534968 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag RG38_RS23585 Protein ID WP_000344800.1
Coordinates 4534994..4535368 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RG38_RS23545 4530039..4530611 + 573 WP_000779831.1 YbaY family lipoprotein -
RG38_RS23550 4530642..4530953 - 312 WP_000409911.1 MGMT family protein -
RG38_RS23560 4531332..4531685 + 354 WP_000878140.1 DUF1428 domain-containing protein -
RG38_RS23565 4531727..4533277 - 1551 WP_001299455.1 cyclic-guanylate-specific phosphodiesterase PdeB -
RG38_RS23570 4533441..4533911 - 471 WP_000136192.1 YlaC family protein -
RG38_RS23575 4534027..4534578 - 552 WP_000102568.1 maltose O-acetyltransferase -
RG38_RS23580 4534750..4534968 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
RG38_RS23585 4534994..4535368 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
RG38_RS23590 4535914..4539063 - 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
RG38_RS23595 4539086..4540279 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T50789 WP_001291435.1 NZ_CP010145:c4534968-4534750 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T50789 NZ_CP010145:c4534968-4534750 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT50789 WP_000344800.1 NZ_CP010145:c4535368-4534994 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT50789 NZ_CP010145:c4535368-4534994 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References