Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 807712..808350 Replicon chromosome
Accession NZ_CP010145
Organism Escherichia coli strain D5

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag RG38_RS04305 Protein ID WP_000813794.1
Coordinates 807712..807888 (+) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag RG38_RS04310 Protein ID WP_001270286.1
Coordinates 807934..808350 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RG38_RS04285 803331..804545 - 1215 WP_001349372.1 BenE family transporter YdcO -
RG38_RS04290 804598..805134 + 537 WP_000429141.1 DNA-binding transcriptional regulator SutR -
RG38_RS04295 805207..807168 + 1962 WP_001307191.1 23S rRNA 5-hydroxycytidine C2501 synthase -
RG38_RS04300 807260..807490 - 231 WP_000494244.1 YncJ family protein -
RG38_RS04305 807712..807888 + 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
RG38_RS04310 807934..808350 + 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
RG38_RS04315 808429..809850 + 1422 WP_073511501.1 PLP-dependent aminotransferase family protein -
RG38_RS26710 809844..810541 - 698 Protein_783 IS1 family transposase -
RG38_RS04340 810597..810965 - 369 Protein_784 ABC transporter ATP-binding protein -
RG38_RS04345 810983..811984 - 1002 Protein_785 ABC transporter substrate-binding protein -
RG38_RS26720 812042..812739 + 698 Protein_786 IS1 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 807260..814350 7090
- flank IS/Tn - - 802026..803291 1265


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T50767 WP_000813794.1 NZ_CP010145:807712-807888 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T50767 NZ_CP010145:807712-807888 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT50767 WP_001270286.1 NZ_CP010145:807934-808350 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT50767 NZ_CP010145:807934-808350 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCGTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAAGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTTGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References