Detailed information of TA system TA050762

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-DnaT
• Location: 4655016..4655538
• Replicon: chromosome
• Accession: NZ_CP010143
• Organism: Escherichia coli strain D4

Toxin (Protein)

• Gene name: relE
• Uniprot ID: G3CAN5
• Locus tag: RG37_RS24380
• Protein ID: WP_000220560.1
• Coordinates: 4655257..4655538 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: G3CAG1
• Locus tag: RG37_RS24375
• Protein ID: WP_000121743.1
• Coordinates: 4655016..4655267 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RG37_RS24340	4650379..4650609	+	231	WP_000051064.1	partitioning protein	-
RG37_RS24345	4650625..4650918	+	294	WP_015060510.1	hypothetical protein	-
RG37_RS24350	4650920..4651888	+	969	WP_077249520.1	IS5-like element IS903B family transposase	-
RG37_RS24355	4651948..4652370	-	423	WP_031942371.1	DNA distortion polypeptide 3	-
RG37_RS24360	4652438..4652884	-	447	WP_001074386.1	hypothetical protein	-
RG37_RS24365	4652924..4653760	-	837	WP_001050931.1	replication initiation protein	-
RG37_RS24375	4655016..4655267	+	252	WP_000121743.1	plasmid stabilization protein	Antitoxin
RG37_RS24380	4655257..4655538	+	282	WP_000220560.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
RG37_RS24385	4655684..4656007	+	324	WP_001181903.1	hypothetical protein	-
RG37_RS24390	4656052..4656297	+	246	WP_000356546.1	hypothetical protein	-
RG37_RS24395	4656287..4656502	+	216	WP_001180116.1	hypothetical protein	-
RG37_RS24400	4656595..4656924	+	330	WP_000866648.1	hypothetical protein	-
RG37_RS27220	4657211..4657360	+	150	WP_000003880.1	hypothetical protein	-
RG37_RS24405	4657373..4657645	+	273	WP_000160399.1	hypothetical protein	-
RG37_RS24410	4657972..4658517	+	546	WP_038976855.1	hypothetical protein	-
RG37_RS24415	4658520..4659677	+	1158	WP_000538023.1	hypothetical protein	-
RG37_RS24420	4660038..4660529	+	492	WP_000872475.1	transcription termination factor NusG	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	floR / aph(3')-Ia / qnrS2 / sul3 / ant(3'')-Ia / cmlA1 / aadA2 / dfrA12	-	4607464..4724856	117392

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 11005.83 Da
• Isoelectric Point: 10.5938

>T50762 WP_000220560.1 NZ_CP010143:4655257-4655538 [Escherichia coli]
MTYELAFDRRALKEWQKLGHTIREQFKKKLAERLENPRVPAARLHGHADRYKIKLRASGYRLVYQVIDEKVVLLVIAVGR
RESSEVYQIADLR

Nucleotide

• Length: 282 bp

>T50762 NZ_CP010143:4655257-4655538 [Escherichia coli]
ATGACCTATGAACTGGCTTTTGACCGTAGAGCACTGAAGGAATGGCAGAAACTCGGCCACACCATCCGTGAACAATTCAA
AAAGAAACTGGCAGAACGGCTGGAAAATCCACGCGTACCCGCAGCCCGGTTACATGGTCATGCTGATCGCTATAAAATCA
AACTTCGTGCATCTGGCTACAGACTTGTATACCAAGTCATTGATGAGAAAGTCGTTTTACTCGTTATTGCCGTTGGAAGA
AGGGAAAGCAGCGAAGTCTATCAGATCGCAGATTTGCGCTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9194.43 Da
• Isoelectric Point: 4.1683

>AT50762 WP_000121743.1 NZ_CP010143:4655016-4655267 [Escherichia coli]
MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCVPPKLYAYYRELAEDAELNAVADERMKNPEIVKVNL
DDL

Nucleotide

• Length: 252 bp

>AT50762 NZ_CP010143:4655016-4655267 [Escherichia coli]
ATGAGCTATCAAATTCTGACAACCACAGCGGCCAGTATTACTGACCTGAAAAAAAATCCTATGGGAACCGTAGCTGAAGG
TGAAGGGGACGCTGTTGCGATCCTGAACCGAAACGAACCGGCGTTCTATTGCGTTCCACCAAAACTTTACGCCTACTATC
GGGAACTCGCTGAAGATGCTGAGTTAAACGCTGTTGCTGATGAGCGCATGAAAAACCCGGAAATTGTGAAGGTTAACCTG
GATGACCTATGA

Structures

Toxin

• AlphaFold DB: G3CAN5

Antitoxin

• AlphaFold DB: A0A3S5I301