Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 1947060..1947698 Replicon chromosome
Accession NZ_CP010143
Organism Escherichia coli strain D4

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag RG37_RS10310 Protein ID WP_000813794.1
Coordinates 1947060..1947236 (+) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag RG37_RS10315 Protein ID WP_001270286.1
Coordinates 1947282..1947698 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RG37_RS10290 1942679..1943893 - 1215 WP_001326689.1 BenE family transporter YdcO -
RG37_RS10295 1943946..1944482 + 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
RG37_RS10300 1944555..1946516 + 1962 WP_001303492.1 23S rRNA 5-hydroxycytidine C2501 synthase -
RG37_RS10305 1946608..1946838 - 231 WP_000494244.1 YncJ family protein -
RG37_RS10310 1947060..1947236 + 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
RG37_RS10315 1947282..1947698 + 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
RG37_RS10320 1947777..1949183 + 1407 WP_000760626.1 PLP-dependent aminotransferase family protein -
RG37_RS10325 1949428..1950573 + 1146 WP_073503305.1 ABC transporter substrate-binding protein -
RG37_RS10330 1950591..1951604 + 1014 WP_073503306.1 ABC transporter ATP-binding protein -
RG37_RS10335 1951605..1952546 + 942 WP_001251304.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T50746 WP_000813794.1 NZ_CP010143:1947060-1947236 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T50746 NZ_CP010143:1947060-1947236 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT50746 WP_001270286.1 NZ_CP010143:1947282-1947698 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT50746 NZ_CP010143:1947282-1947698 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References