Detailed information of TA system TA050722

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/-
• Location: 52884..53260
• Replicon: plasmid A
• Accession: NZ_CP010141
• Organism: Escherichia coli strain D3

Toxin (Protein)

• Gene name: parE
• Uniprot ID: A0A075MDK3
• Locus tag: RG36_RS26555
• Protein ID: WP_000969988.1
• Coordinates: 53078..53260 (+)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: -
• Locus tag: RG36_RS26550
• Protein ID: WP_001324224.1
• Coordinates: 52884..53081 (+)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RG36_RS29375	48144..48380	+	237	WP_001323890.1	colicin V immunity protein	-
RG36_RS26530	48358..48669	+	312	WP_001259758.1	colicin V	-
RG36_RS26535	48839..50953	-	2115	WP_001183604.1	peptidase domain-containing ABC transporter	-
RG36_RS26540	50928..52202	-	1275	WP_000489609.1	HlyD family secretion protein	-
RG36_RS26550	52884..53081	+	198	WP_001324224.1	toxin-antitoxin system protein	Antitoxin
RG36_RS26555	53078..53260	+	183	WP_000969988.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
RG36_RS26560	53359..53667	+	309	WP_000377483.1	hypothetical protein	-
RG36_RS26570	54227..55261	+	1035	WP_001190234.1	3-deoxy-7-phosphoheptulonate synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aph(6)-Id / aph(3'')-Ib / sul2 / mph(A) / blaTEM-1B / tet(B) / catA1 / aadA5 / qacE / sul1 / blaCTX-M-55 / sitABCD	iroN / iroE / iroD / iroC / iroB / iucA / iucB / iucC / iucD / iutA	1..174041	174041

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Small toxic protein BsrE-like (30-58)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6931.07 Da
• Isoelectric Point: 4.5349

>T50722 WP_000969988.1 NZ_CP010141:53078-53260 [Escherichia coli]
MMEIFWTMLASQDRKRIREYIAEQNLIAAIELDERIGCSASLLFSLSFISVQVHDNIITV

Nucleotide

• Length: 183 bp

>T50722 NZ_CP010141:53078-53260 [Escherichia coli]
ATGATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGAT
AGCCGCCATTGAACTGGACGAACGGATTGGTTGTTCAGCCAGCCTCCTGTTCTCCCTTTCTTTTATCAGTGTACAGGTGC
ATGACAACATAATCACGGTATGA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 7658.53 Da
• Isoelectric Point: 7.2046

>AT50722 WP_001324224.1 NZ_CP010141:52884-53081 [Escherichia coli]
MDRNGSQLIRDFMRQTVERQHNTWFRDQVEAGRQQLERGDVLPHDMVESSAAAWRDEMSRKVAGK

Nucleotide

• Length: 198 bp

>AT50722 NZ_CP010141:52884-53081 [Escherichia coli]
ATGGACCGTAATGGCTCTCAGTTAATCCGGGATTTTATGCGCCAGACCGTGGAACGGCAGCATAATACCTGGTTCCGTGA
CCAGGTTGAGGCAGGACGTCAGCAACTCGAGCGCGGCGATGTGCTTCCCCATGACATGGTCGAATCTTCCGCTGCTGCAT
GGCGGGATGAAATGAGCAGGAAAGTCGCCGGTAAATGA

Structures

Toxin

• AlphaFold DB: A0A075MDK3