Detailed information of TA system TA050715

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 3243576..3244201
• Replicon: chromosome
• Accession: NZ_CP010140
• Organism: Escherichia coli strain D3

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: RG36_RS17025
• Protein ID: WP_000911330.1
• Coordinates: 3243576..3243974 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: RG36_RS17030
• Protein ID: WP_000450524.1
• Coordinates: 3243974..3244201 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RG36_RS17005	3239454..3239654	+	201	WP_000383836.1	YpfN family protein	-
RG36_RS17010	3239764..3240462	-	699	WP_000679812.1	esterase	-
RG36_RS17015	3240536..3242551	-	2016	WP_069354268.1	tRNA cytosine(34) acetyltransferase TmcA	-
RG36_RS17020	3242566..3243429	-	864	WP_001267507.1	neutral zinc metallopeptidase	-
RG36_RS17025	3243576..3243974	-	399	WP_000911330.1	tRNA(fMet)-specific endonuclease VapC	Toxin
RG36_RS17030	3243974..3244201	-	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
RG36_RS17035	3244355..3245068	-	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
RG36_RS17045	3245281..3246315	-	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
RG36_RS17050	3246332..3247210	-	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
RG36_RS17055	3247356..3247928	+	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
RG36_RS17060	3247928..3248398	+	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T50715 WP_000911330.1 NZ_CP010140:c3243974-3243576 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T50715 NZ_CP010140:c3243974-3243576 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT50715 WP_000450524.1 NZ_CP010140:c3244201-3243974 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT50715 NZ_CP010140:c3244201-3243974 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33