Detailed information of TA system TA050697

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 19450..20093
• Replicon: plasmid B
• Accession: NZ_CP010139
• Organism: Escherichia coli strain D2

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: RG35_RS25680
• Protein ID: WP_077900217.1
• Coordinates: 19450..19866 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: D2WFK3
• Locus tag: RG35_RS25685
• Protein ID: WP_001261287.1
• Coordinates: 19863..20093 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RG35_RS25655	14812..15294	+	483	WP_032182006.1	hypothetical protein	-
RG35_RS25660	15584..15931	+	348	WP_000142441.1	hypothetical protein	-
RG35_RS25665	15949..16539	-	591	WP_020219166.1	hypothetical protein	-
RG35_RS25670	16536..16796	-	261	WP_000371882.1	hypothetical protein	-
RG35_RS25675	17150..19288	+	2139	WP_000350635.1	AAA family ATPase	-
RG35_RS25680	19450..19866	-	417	WP_077900217.1	type II toxin-antitoxin system VapC family toxin	Toxin
RG35_RS25685	19863..20093	-	231	WP_001261287.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
RG35_RS25690	20399..23518	+	3120	WP_001553851.1	hypothetical protein	-
RG35_RS25695	24085..24791	-	707	Protein_31	IS1 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	espC	1..27342	27342
	-	flank	IS/Tn	-	-	24085..24597	512

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15227.68 Da
• Isoelectric Point: 7.7805

>T50697 WP_077900217.1 NZ_CP010139:c19866-19450 [Escherichia coli]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDEFCARLDAILPWDRA
AVDATTKIKVALRLTGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK

Nucleotide

• Length: 417 bp

>T50697 NZ_CP010139:c19866-19450 [Escherichia coli]
GTGAACAAGACTTATATGCTTGATACCTGTATCTGCTCGTTCATCATGCGTGAACAGCCAGAAGCCGTCCTGAAGCGTCT
GGAGCAGGCGGTGCTACGCGGCCACCGTATAGTGGTCTCGGCCATCACCTACTCTGAAATGCGCTTCGGGGCTACCGGCC
CAAAGGCCTCACCACGTCACGTCCAACTGGTCGATGAATTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCC
GCAGTAGACGCCACCACGAAGATTAAAGTGGCACTGCGGCTGACCGGGACGCCGATCGGCCCGAACGACACGGCGATTGC
CGGGCACGCCATCGCCGCCGGGGCGATACTGGTAACGAATAATACAAGAGAATTTGAGCGGGTGCCTGACCTGGTGCTGG
AAGACTGGGTGAAGTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8815.90 Da
• Isoelectric Point: 4.3775

>AT50697 WP_001261287.1 NZ_CP010139:c20093-19863 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLEYEKADPDFMAEREDVVSDEGRFNL

Nucleotide

• Length: 231 bp

>AT50697 NZ_CP010139:c20093-19863 [Escherichia coli]
ATGAGAACCGTATCGATATTTAAAAATGGCAACAACCGCGCCATCCGTCTGCCTCGTGATCTGGATTTTGAGGGGGTGAG
CGAGCTCGAGATCGTCCGGGAAGGGGACAGCATCATCCTGCGCCCCGTCCGGCCAACCTGGGGATCATTCCTAGAGTACG
AAAAAGCCGATCCAGATTTTATGGCGGAGCGCGAGGATGTTGTCAGCGACGAAGGGCGATTTAACCTGTGA

Structures

Antitoxin

• AlphaFold DB: A0A7U9QFC4