50696

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) parDE/RelE-RHH
Location 14174..14721 Replicon plasmid B
Accession NZ_CP010139
Organism Escherichia coli strain D2

Toxin (Protein)


Gene name parE Uniprot ID E9LLH3
Locus tag RG35_RS25650 Protein ID WP_000969996.1
Coordinates 14440..14721 (+) Length 94 a.a.

Antitoxin (Protein)


Gene name parD Uniprot ID E1QMW3
Locus tag RG35_RS25645 Protein ID WP_000079941.1
Coordinates 14174..14443 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RG35_RS26775 9951..10019 + 69 Protein_13 hypothetical protein -
RG35_RS25590 10019..10963 - 945 Protein_14 translesion error-prone DNA polymerase V subunit UmuC -
RG35_RS25595 10963..11172 - 210 Protein_15 peptidase -
RG35_RS26780 11148..11258 + 111 Protein_16 hypothetical protein -
RG35_RS25605 11254..11601 + 348 Protein_17 DUF2726 domain-containing protein -
RG35_RS25610 11841..12095 + 255 WP_000083830.1 replication regulatory protein RepA -
RG35_RS25620 12333..12407 + 75 WP_001375168.1 RepA leader peptide Tap -
RG35_RS25625 12400..13257 + 858 WP_073544452.1 incFII family plasmid replication initiator RepA -
RG35_RS25645 14174..14443 + 270 WP_000079941.1 hypothetical protein Antitoxin
RG35_RS25650 14440..14721 + 282 WP_000969996.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
RG35_RS25655 14812..15294 + 483 WP_032182006.1 hypothetical protein -
RG35_RS25660 15584..15931 + 348 WP_000142441.1 hypothetical protein -
RG35_RS25665 15949..16539 - 591 WP_020219166.1 hypothetical protein -
RG35_RS25670 16536..16796 - 261 WP_000371882.1 hypothetical protein -
RG35_RS25675 17150..19288 + 2139 WP_000350635.1 AAA family ATPase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - espC 1..27342 27342


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-92)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 10893.57 Da        Isoelectric Point: 8.4615

>T50696 WP_000969996.1 NZ_CP010139:14440-14721 [Escherichia coli]
MMEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKV
YILRVLHTAQKWP

Download         Length: 282 bp

>T50696 NZ_CP010139:14440-14721 [Escherichia coli]
ATGATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGAT
GGCCGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAGCCTAGCCGGACAGCCGTATAAAGGCCGCAACGGTC
GTGTGGAAGGTACCCGGGAACTGGTTATTCACCCGCATTTTGTTCTGGTTTATGAGGTCGACAGCCAGTGGGGAAAAGTG
TATATCCTGCGTGTATTGCATACTGCACAGAAGTGGCCATAG

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10252.58 Da        Isoelectric Point: 7.2057

>AT50696 WP_000079941.1 NZ_CP010139:14174-14443 [Escherichia coli]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVEAGRQQLERGDVLPHDMVESSAAAWRD
EMSRKVAGK

Download         Length: 270 bp

>AT50696 NZ_CP010139:14174-14443 [Escherichia coli]
ATGGCACAGGTTAATATGAGTTTAAGAATCGACGCTGAACTGAAGGATGCTTTTATGGCCGCTGCAAAAAGCATGGACCG
TAATGGCTCTCAGTTAATCCGGGATTTTATGCGCCAGACCGTTGAACGGCAGCATAATACCTGGTTCCGTGACCAGGTTG
AGGCAGGACGTCAGCAACTCGAGCGCGGCGATGTGCTTCCCCATGACATGGTCGAATCTTCCGCTGCTGCATGGCGGGAT
GAAATGAGCAGGAAGGTTGCCGGTAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB E9LLH3

Antitoxin

Source ID Structure
AlphaFold DB A0A629TXC5

References