Detailed information of TA system TA050690

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yjhX-yjhQ/YjhX-GNAT
• Location: 4236649..4237463
• Replicon: chromosome
• Accession: NZ_CP010137
• Organism: Escherichia coli strain D2

Toxin (Protein)

• Gene name: yjhX
• Uniprot ID: A0A6C9SL90
• Locus tag: RG35_RS22215
• Protein ID: WP_001054375.1
• Coordinates: 4236649..4236906 (+)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: yjhQ
• Uniprot ID: U9Z4B8
• Locus tag: RG35_RS22220
• Protein ID: WP_001309181.1
• Coordinates: 4236918..4237463 (+)
• Length: 182 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RG35_RS22190	4231937..4233043	+	1107	WP_001299704.1	N-acetylneuraminate epimerase	-
RG35_RS22195	4233108..4234088	+	981	WP_000991455.1	9-O-acetyl-N-acetylneuraminic acid deacetylase	-
RG35_RS22205	4234671..4235911	-	1241	Protein_3992	helicase YjhR	-
RG35_RS26665	4236027..4236272	+	246	Protein_3993	GNAT family N-acetyltransferase	-
RG35_RS22215	4236649..4236906	+	258	WP_001054375.1	hypothetical protein	Toxin
RG35_RS22220	4236918..4237463	+	546	WP_001309181.1	N-acetyltransferase	Antitoxin
RG35_RS22225	4237519..4238265	+	747	WP_000354252.1	class I SAM-dependent methyltransferase	-
RG35_RS26175	4238434..4238652	+	219	Protein_3997	hypothetical protein	-
RG35_RS27225	4238690..4238806	+	117	Protein_3998	VOC family protein	-
RG35_RS22235	4239051..4240172	+	1122	WP_000010831.1	M42 family metallopeptidase	-
RG35_RS22240	4240169..4240447	+	279	WP_000722973.1	PTS sugar transporter subunit IIB	-
RG35_RS22245	4240459..4241772	+	1314	WP_000460845.1	galactitol-specific PTS transporter subunit IIC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fimB	4229256..4245687	16431

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Putative toxin of bacterial toxin-antitoxin pair (1-85)

Antitoxin

• GNAT domain (18-121)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 9768.31 Da
• Isoelectric Point: 11.0090

>T50690 WP_001054375.1 NZ_CP010137:4236649-4236906 [Escherichia coli]
MNLSRQEQHTLHVLAKGRRIAHVRDSSGRVTSVECYSREGLLLTDCTLAVFKKLKTKKLIKSVNGQPYRINTTELNKVRA
QFDNR

Nucleotide

• Length: 258 bp

>T50690 NZ_CP010137:4236649-4236906 [Escherichia coli]
ATGAATTTATCCCGTCAGGAACAACATACCTTACACGTTCTCGCTAAAGGTAGACGTATTGCGCACGTCCGCGATTCTTC
AGGCCGCGTCACTTCCGTTGAATGCTACAGCCGCGAAGGGCTGTTGCTGACCGACTGCACGCTCGCCGTCTTCAAAAAAC
TCAAAACCAAAAAACTTATCAAGTCCGTCAATGGCCAGCCCTATCGAATCAACACCACCGAGCTGAATAAAGTTCGCGCT
CAGTTCGATAATCGCTAA

Antitoxin

Protein

• Length: 182 a.a.
• Molecular weight: 19938.86 Da
• Isoelectric Point: 6.3277

>AT50690 WP_001309181.1 NZ_CP010137:4236918-4237463 [Escherichia coli]
ITVHHFTFHITDKSDASDIREVETRAFGFSKEADLVASLLEDESARPALSLLARYEGKAVGHILFTRATFKGEMDSPLMH
ILAPLAVIPEYQGMGVGGRLIRTGIEHLRLMGCQTVFVLGHATYYPRHGFEPCAGDKGYPAPYPIPEEHKACWMMQSLTA
QPMTLTGHIRCADPDETGALT

Nucleotide

• Length: 546 bp

>AT50690 NZ_CP010137:4236918-4237463 [Escherichia coli]
ATAACAGTACACCACTTCACTTTTCATATCACAGATAAAAGTGATGCCAGTGATATCCGGGAAGTCGAAACCCGCGCATT
TGGCTTCAGCAAAGAAGCCGATTTAGTGGCCTCTTTACTTGAGGATGAAAGCGCGCGCCCCGCGTTATCGCTGCTAGCCC
GGTATGAAGGCAAAGCCGTCGGGCATATTTTATTTACCCGGGCAACCTTCAAAGGAGAAATGGATTCACCGTTGATGCAC
ATCCTCGCTCCCCTGGCGGTCATACCGGAATACCAAGGGATGGGCGTGGGAGGACGGTTGATACGCACTGGCATCGAGCA
CCTGAGGTTGATGGGCTGCCAGACGGTTTTTGTTCTCGGCCACGCCACCTATTACCCCCGACATGGGTTCGAACCCTGTG
CCGGAGACAAAGGCTATCCGGCACCTTATCCCATCCCGGAGGAGCACAAAGCCTGCTGGATGATGCAATCACTCACCGCT
CAGCCCATGACCCTGACCGGCCACATCCGGTGCGCAGACCCTGATGAAACTGGAGCACTGACGTGA

Structures

Toxin

• AlphaFold DB: A0A6C9SL90

Antitoxin

• AlphaFold DB: U9Z4B8