50684

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2658818..2659456 Replicon chromosome
Accession NZ_CP010137
Organism Escherichia coli strain D2

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag RG35_RS13825 Protein ID WP_000813794.1
Coordinates 2659280..2659456 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag RG35_RS13820 Protein ID WP_001270286.1
Coordinates 2658818..2659234 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RG35_RS13800 2653970..2654911 - 942 WP_001251313.1 ABC transporter permease -
RG35_RS13805 2654912..2655925 - 1014 WP_000220396.1 ABC transporter ATP-binding protein -
RG35_RS13810 2655943..2657088 - 1146 WP_000047424.1 ABC transporter substrate-binding protein -
RG35_RS13815 2657333..2658739 - 1407 WP_000760626.1 PLP-dependent aminotransferase family protein -
RG35_RS13820 2658818..2659234 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
RG35_RS13825 2659280..2659456 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
RG35_RS13830 2659678..2659908 + 231 WP_000494244.1 YncJ family protein -
RG35_RS13835 2660000..2661961 - 1962 WP_073544264.1 23S rRNA 5-hydroxycytidine C2501 synthase -
RG35_RS13840 2662034..2662570 - 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
RG35_RS13845 2662623..2663837 + 1215 WP_001326689.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T50684 WP_000813794.1 NZ_CP010137:c2659456-2659280 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T50684 NZ_CP010137:c2659456-2659280 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT50684 WP_001270286.1 NZ_CP010137:c2659234-2658818 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT50684 NZ_CP010137:c2659234-2658818 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTGCCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References