Detailed information of TA system TA050672

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 1016480..1017312
• Replicon: chromosome
• Accession: NZ_CP010137
• Organism: Escherichia coli strain D2

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: S1PD64
• Locus tag: RG35_RS05275
• Protein ID: WP_000854753.1
• Coordinates: 1016480..1016854 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: S1NM52
• Locus tag: RG35_RS05280
• Protein ID: WP_001295723.1
• Coordinates: 1016944..1017312 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RG35_RS05245	1011593..1012741	-	1149	WP_073544095.1	capsule biosynthesis protein	-
RG35_RS05250	1012813..1013796	-	984	WP_001401108.1	KpsF/GutQ family sugar-phosphate isomerase	-
RG35_RS05255	1014607..1014777	-	171	Protein_948	IS110 family transposase	-
RG35_RS05260	1015120..1015689	-	570	WP_001560692.1	DUF4942 domain-containing protein	-
RG35_RS05265	1015786..1015983	-	198	WP_000839281.1	DUF957 domain-containing protein	-
RG35_RS05270	1015995..1016483	-	489	WP_000777545.1	hypothetical protein	-
RG35_RS05275	1016480..1016854	-	375	WP_000854753.1	TA system toxin CbtA family protein	Toxin
RG35_RS05280	1016944..1017312	-	369	WP_001295723.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
RG35_RS05290	1017475..1017696	-	222	WP_000692311.1	DUF987 domain-containing protein	-
RG35_RS05295	1017759..1018235	-	477	WP_073544096.1	RadC family protein	-
RG35_RS05300	1018251..1018736	-	486	WP_000214398.1	antirestriction protein	-
RG35_RS05305	1018827..1019645	-	819	WP_073544097.1	DUF945 domain-containing protein	-
RG35_RS05310	1019735..1019968	-	234	WP_001278283.1	DUF905 family protein	-
RG35_RS05315	1019974..1020651	-	678	WP_001097301.1	hypothetical protein	-
RG35_RS05320	1020799..1021479	-	681	WP_001282919.1	WYL domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 14005.00 Da
• Isoelectric Point: 8.2905

>T50672 WP_000854753.1 NZ_CP010137:c1016854-1016480 [Escherichia coli]
MKTLPDTHVREASSCPSPVTIWQTLLSRLLGQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEKR

Nucleotide

• Length: 375 bp

>T50672 NZ_CP010137:c1016854-1016480 [Escherichia coli]
ATGAAAACATTACCCGATACACACGTACGGGAGGCATCGAGCTGCCCGTCTCCCGTCACCATCTGGCAGACTCTGCTCTC
CCGACTACTGGGCCAGCATTACGGCCTTACACTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACTTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGCATCCGGAGAAACGATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13592.40 Da
• Isoelectric Point: 6.6255

>AT50672 WP_001295723.1 NZ_CP010137:c1017312-1016944 [Escherichia coli]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYPLDQAFPLLMKQLKLMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT50672 NZ_CP010137:c1017312-1016944 [Escherichia coli]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGAGATGCGGATGCGTACCCTCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGAAATTAATGCTCACCAGTGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCTGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A7U9LVU0

Antitoxin

• AlphaFold DB: S1NM52