Detailed information of TA system TA050668

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 31611..32146
• Replicon: plasmid B
• Accession: NZ_CP010136
• Organism: Escherichia coli strain D1

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: RG34_RS27665
• Protein ID: WP_001270421.1
• Coordinates: 31611..31898 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: L4J0V6
• Locus tag: RG34_RS27670
• Protein ID: WP_001132895.1
• Coordinates: 31895..32146 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RG34_RS27605	26716..26994	-	279	WP_123059140.1	antirestriction protein	-
RG34_RS29175	27373..28149	-	777	WP_073514799.1	hypothetical protein	-
RG34_RS27620	28195..28629	-	435	WP_073514800.1	DUF1380 family protein	-
RG34_RS27625	28641..28862	-	222	WP_073514801.1	hypothetical protein	-
RG34_RS27630	28863..29546	-	684	WP_073514802.1	DNA methylase	-
RG34_RS27640	29623..29937	-	315	WP_073514814.1	hypothetical protein	-
RG34_RS27645	29881..30261	+	381	Protein_41	transposase	-
RG34_RS27650	30378..30716	-	339	WP_106426746.1	IS66 family insertion sequence element accessory protein TnpB	-
RG34_RS27655	30713..31138	-	426	WP_000422702.1	IS66 family insertion sequence hypothetical protein	-
RG34_RS27660	31189..31609	-	421	Protein_44	DDE-type integrase/transposase/recombinase	-
RG34_RS27665	31611..31898	-	288	WP_001270421.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
RG34_RS27670	31895..32146	-	252	WP_001132895.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
RG34_RS27675	32320..32532	-	213	WP_000402944.1	hypothetical protein	-
RG34_RS27685	32906..34111	+	1206	WP_000817642.1	AAA family ATPase	-
RG34_RS27690	34108..35070	+	963	WP_000756329.1	ParB/RepB/Spo0J family partition protein	-
RG34_RS27695	35210..36371	-	1162	Protein_50	DUF4113 domain-containing protein	-
RG34_RS29180	36252..36668	+	417	Protein_51	DUF1281 domain-containing protein	-
RG34_RS27705	36672..36977	+	306	WP_032163448.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	cif	1..55203	55203

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-83)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11244.27 Da
• Isoelectric Point: 10.4703

>T50668 WP_001270421.1 NZ_CP010136:c31898-31611 [Escherichia coli]
MRYQVKFREDALKEWQKLDKTIQQQFAKKLKKCCDNPHIPSAKLRGIKDCYKIKLRASGFRLVYQVIDEQLIIAVVAVGK
RERSDVYNLASERMR

Nucleotide

• Length: 288 bp

>T50668 NZ_CP010136:c31898-31611 [Escherichia coli]
ATGAGGTATCAGGTAAAATTCAGGGAAGATGCGCTGAAAGAGTGGCAAAAACTGGACAAGACTATTCAGCAACAGTTTGC
GAAAAAGCTAAAAAAGTGCTGTGACAATCCGCATATTCCTTCCGCAAAACTGCGCGGGATAAAGGACTGCTACAAAATAA
AATTACGTGCGTCAGGTTTTCGCCTAGTCTATCAGGTGATTGACGAACAATTAATTATCGCTGTTGTAGCTGTGGGTAAG
CGTGAGCGCAGTGACGTTTATAATCTTGCCAGTGAAAGAATGAGATAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9178.47 Da
• Isoelectric Point: 4.2108

>AT50668 WP_001132895.1 NZ_CP010136:c32146-31895 [Escherichia coli]
MPNIILSDTSASVSELKKNPMATVSAGDGFPVAILNRNQPAFYCVPAELYEKMLDALDDQELVKLVAERSNQPLHDVDLD
KYL

Nucleotide

• Length: 252 bp

>AT50668 NZ_CP010136:c32146-31895 [Escherichia coli]
ATGCCTAACATTATTCTCAGTGATACAAGCGCCAGTGTCAGCGAGCTGAAGAAAAATCCGATGGCGACAGTCAGCGCCGG
TGATGGTTTCCCGGTCGCTATCCTGAACCGTAATCAGCCTGCTTTCTACTGTGTACCCGCAGAGCTGTACGAAAAGATGC
TCGATGCCCTGGACGATCAGGAGCTGGTTAAACTGGTAGCCGAACGCAGCAACCAACCGCTGCATGATGTAGACCTAGAT
AAATACCTATGA

Structures

Antitoxin

• AlphaFold DB: L4J0V6