Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 9049..9574 Replicon plasmid B
Accession NZ_CP010136
Organism Escherichia coli strain D1

Toxin (Protein)


Gene name ccdB Uniprot ID -
Locus tag RG34_RS27435 Protein ID WP_073514788.1
Coordinates 9049..9354 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag RG34_RS27440 Protein ID WP_000813634.1
Coordinates 9356..9574 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RG34_RS27380 4455..4988 - 534 Protein_5 IS3 family transposase -
RG34_RS27415 7419..7793 - 375 Protein_8 transposase -
RG34_RS28420 7876..8100 - 225 Protein_9 IS66 family insertion sequence element accessory protein TnpB -
RG34_RS27430 8241..9048 - 808 Protein_10 phage integrase family protein -
RG34_RS27435 9049..9354 - 306 WP_073514788.1 type II toxin-antitoxin system toxin CcdB Toxin
RG34_RS27440 9356..9574 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
RG34_RS27450 10169..10438 + 270 WP_000343793.1 hypothetical protein -
RG34_RS27455 10426..11022 + 597 WP_059219358.1 hypothetical protein -
RG34_RS27470 11799..12776 + 978 WP_000361615.1 RepB family plasmid replication initiator protein -
RG34_RS27475 13061..13801 - 741 WP_073514789.1 tyrosine-type recombinase/integrase -
RG34_RS27480 13922..14077 - 156 WP_097760941.1 transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - cif 1..55203 55203


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11722.47 Da        Isoelectric Point: 5.7116

>T50667 WP_073514788.1 NZ_CP010136:c9354-9049 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEE
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T50667 NZ_CP010136:c9354-9049 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTCCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGCGCCCGCCTGCTGTCAGACAAAGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGAG
GCCGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT50667 WP_000813634.1 NZ_CP010136:c9574-9356 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT50667 NZ_CP010136:c9574-9356 [Escherichia coli]
ATGAAGCAGCGCATTACAGTCACCGTCGACAGTGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACCACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCAGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References