Detailed information of TA system TA050642

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 5403682..5404517
• Replicon: chromosome
• Accession: NZ_CP010133
• Organism: Escherichia coli strain C11

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: F2VI91
• Locus tag: RG33_RS28035
• Protein ID: WP_000854748.1
• Coordinates: 5403682..5404059 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: RG33_RS28040
• Protein ID: WP_040089920.1
• Coordinates: 5404149..5404517 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RG33_RS28000	5399262..5399816	-	555	WP_001001902.1	molecular chaperone YcdY	-
RG33_RS28005	5399840..5400577	-	738	WP_000283664.1	zinc-binding phosphatase	-
RG33_RS28010	5400632..5401570	-	939	WP_000351317.1	glyoxylate/hydroxypyruvate reductase GhrA	-
RG33_RS28020	5402041..5402883	-	843	WP_047634698.1	DUF4942 domain-containing protein	-
RG33_RS28025	5402980..5403177	-	198	WP_000772024.1	DUF957 domain-containing protein	-
RG33_RS28030	5403197..5403685	-	489	WP_032215286.1	hypothetical protein	-
RG33_RS28035	5403682..5404059	-	378	WP_000854748.1	TA system toxin CbtA family protein	Toxin
RG33_RS28040	5404149..5404517	-	369	WP_040089920.1	type IV toxin-antitoxin system toxin CbtA	Antitoxin
RG33_RS28045	5404597..5404818	-	222	WP_000692323.1	DUF987 domain-containing protein	-
RG33_RS28050	5404881..5405357	-	477	WP_001186726.1	RadC family protein	-
RG33_RS28055	5405373..5405858	-	486	WP_000849582.1	antirestriction protein	-
RG33_RS28060	5405913..5406731	-	819	WP_001234620.1	DUF945 domain-containing protein	-
RG33_RS28070	5406831..5407064	-	234	WP_001119729.1	DUF905 family protein	-
RG33_RS28075	5407143..5407598	-	456	WP_000581504.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	csgC / csgA / csgB / csgD / csgE / csgF / csgG	5394163..5405858	11695

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (19-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14179.16 Da
• Isoelectric Point: 7.7761

>T50642 WP_000854748.1 NZ_CP010133:c5404059-5403682 [Escherichia coli]
MKTLPDTHVREASSCPSPVTIWQTLLSRLLGQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPEF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYQEAKR

Nucleotide

• Length: 378 bp

>T50642 NZ_CP010133:c5404059-5403682 [Escherichia coli]
ATGAAAACATTACCCGATACACACGTACGGGAGGCATCGAGCTGCCCGTCTCCCGTCACCATCTGGCAGACTCTGCTCTC
CCGACTACTGGGCCAGCATTACGGCCTTACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAATTTTCTCGTGGAAAAATACGCGTTGGTGCGTACCGACCAGCCGGAATTC
AGTGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCCGGGCGACTGGCCTGATGACCCGCGA
CAATTACCGAACGGTAAATAACATTACCCTGGGTAAGTATCAGGAGGCGAAACGATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13529.45 Da
• Isoelectric Point: 6.6290

>AT50642 WP_040089920.1 NZ_CP010133:c5404517-5404149 [Escherichia coli]
VSDTLPGTILPDDNHDRLWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLGSGGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT50642 NZ_CP010133:c5404517-5404149 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAATACTTCCCGACGACAATCACGACCGCCTCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGTCTGACCTGCAAAGCTGACACTCTCGGTAGCGG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: F2VI91