Detailed information of TA system TA050638

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 4876332..4876858
• Replicon: chromosome
• Accession: NZ_CP010133
• Organism: Escherichia coli strain C11

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1EXL1
• Locus tag: RG33_RS25230
• Protein ID: WP_000323025.1
• Coordinates: 4876571..4876858 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: S1F6D3
• Locus tag: RG33_RS25225
• Protein ID: WP_000534858.1
• Coordinates: 4876332..4876571 (+)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RG33_RS25180	4871666..4872022	+	357	WP_001374839.1	class I SAM-dependent methyltransferase	-
RG33_RS29400	4872633..4872920	+	288	Protein_4508	hypothetical protein	-
RG33_RS25205	4873689..4875030	+	1342	Protein_4509	ISNCY family transposase	-
RG33_RS25210	4875467..4875799	-	333	WP_001317460.1	FlxA-like family protein	-
RG33_RS25220	4876002..4876307	-	306	WP_001326990.1	hypothetical protein	-
RG33_RS25225	4876332..4876571	+	240	WP_000534858.1	type II toxin-antitoxin system antitoxin RelB	Antitoxin
RG33_RS25230	4876571..4876858	+	288	WP_000323025.1	type II toxin-antitoxin system mRNA interferase RelE	Toxin
RG33_RS25235	4876930..4877085	+	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	-
RG33_RS25240	4877302..4877553	+	252	WP_000980994.1	hypothetical protein	-
RG33_RS25245	4877620..4877898	+	279	WP_040090315.1	hypothetical protein	-
RG33_RS25250	4877900..4878949	+	1050	WP_001265198.1	DUF968 domain-containing protein	-
RG33_RS25255	4878963..4879715	+	753	WP_040090316.1	antitermination protein	-
RG33_RS29000	4879994..4880083	-	90	WP_120795389.1	hypothetical protein	-
RG33_RS25260	4880138..4880350	-	213	WP_000087756.1	cold shock-like protein CspF	-
RG33_RS25265	4880651..4880866	+	216	WP_000066484.1	cold shock-like protein CspB	-
RG33_RS29405	4881230..4881400	-	171	WP_211180497.1	putative zinc-binding protein YnfU	-
RG33_RS25270	4881620..4881835	+	216	WP_000839590.1	phage lysis protein EssD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	4851467..4920331	68864

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-79)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11225.22 Da
• Isoelectric Point: 10.1967

>T50638 WP_000323025.1 NZ_CP010133:4876571-4876858 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Nucleotide

• Length: 288 bp

>T50638 NZ_CP010133:4876571-4876858 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9071.48 Da
• Isoelectric Point: 4.5230

>AT50638 WP_000534858.1 NZ_CP010133:4876332-4876571 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Nucleotide

• Length: 240 bp

>AT50638 NZ_CP010133:4876332-4876571 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Structures

Toxin

• PDB: 4V7K
• PDB: 4V7J
• PDB: 4FXI
• PDB: 4FXE
• PDB: 2KC8
• PDB: 4FXH
• PDB: 2KC9

Antitoxin

• PDB: 4FXE
• PDB: 2KC8
• PDB: 2K29
• AlphaFold DB: A0A4V1CTS8