Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3455532..3456186 Replicon chromosome
Accession NZ_CP010133
Organism Escherichia coli strain C11

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag RG33_RS17840 Protein ID WP_000244777.1
Coordinates 3455779..3456186 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag RG33_RS17835 Protein ID WP_000354046.1
Coordinates 3455532..3455798 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RG33_RS17810 3450701..3451444 + 744 WP_000951964.1 SDR family oxidoreductase -
RG33_RS17815 3451501..3452934 - 1434 WP_001307385.1 6-phospho-beta-glucosidase BglA -
RG33_RS17820 3452979..3453290 + 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
RG33_RS17825 3453454..3454113 + 660 WP_000250274.1 hemolysin III family protein -
RG33_RS17830 3454309..3455289 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
RG33_RS17835 3455532..3455798 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
RG33_RS17840 3455779..3456186 + 408 WP_000244777.1 toxin CptA Toxin
RG33_RS17845 3456226..3456747 - 522 WP_001055874.1 flavodoxin FldB -
RG33_RS17850 3456859..3457755 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
RG33_RS17855 3457780..3458490 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
RG33_RS17860 3458496..3460229 + 1734 WP_000813212.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T50626 WP_000244777.1 NZ_CP010133:3455779-3456186 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T50626 NZ_CP010133:3455779-3456186 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT50626 WP_000354046.1 NZ_CP010133:3455532-3455798 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT50626 NZ_CP010133:3455532-3455798 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References