Detailed information of TA system TA050625

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3243229..3244061
• Replicon: chromosome
• Accession: NZ_CP010133
• Organism: Escherichia coli strain C11

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: RG33_RS16660
• Protein ID: WP_073487848.1
• Coordinates: 3243229..3243603 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: RG33_RS16665
• Protein ID: WP_040089920.1
• Coordinates: 3243693..3244061 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RG33_RS16625	3238293..3238898	+	606	WP_001255030.1	type II secretion system minor pseudopilin GspJ	-
RG33_RS16630	3238895..3239872	+	978	WP_000633209.1	type II secretion system minor pseudopilin GspK	-
RG33_RS16635	3239869..3241047	+	1179	WP_000094972.1	type II secretion system protein GspL	-
RG33_RS16640	3241049..3241585	+	537	WP_000942770.1	GspM family type II secretion system protein YghD	-
RG33_RS16645	3241866..3242438	-	573	WP_001290246.1	DUF4942 domain-containing protein	-
RG33_RS16650	3242535..3242732	-	198	WP_000839281.1	DUF957 domain-containing protein	-
RG33_RS16655	3242744..3243232	-	489	WP_000777541.1	hypothetical protein	-
RG33_RS16660	3243229..3243603	-	375	WP_073487848.1	TA system toxin CbtA family protein	Toxin
RG33_RS16665	3243693..3244061	-	369	WP_040089920.1	type IV toxin-antitoxin system toxin CbtA	Antitoxin
RG33_RS16670	3244141..3244362	-	222	WP_000692323.1	DUF987 domain-containing protein	-
RG33_RS16675	3244425..3244901	-	477	WP_001186726.1	RadC family protein	-
RG33_RS16680	3244917..3245402	-	486	WP_000849582.1	antirestriction protein	-
RG33_RS16685	3245457..3246275	-	819	WP_001234620.1	DUF945 domain-containing protein	-
RG33_RS16695	3246375..3246608	-	234	WP_001119729.1	DUF905 family protein	-
RG33_RS16700	3246687..3247142	-	456	WP_000581504.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (19-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 14004.02 Da
• Isoelectric Point: 8.7453

>T50625 WP_073487848.1 NZ_CP010133:c3243603-3243229 [Escherichia coli]
MKTLPNTHVREASSCPSPVTIWQTLLSRLLGQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEKR

Nucleotide

• Length: 375 bp

>T50625 NZ_CP010133:c3243603-3243229 [Escherichia coli]
ATGAAAACATTACCCAATACACACGTACGGGAGGCATCGAGCTGCCCGTCTCCCGTCACCATCTGGCAGACTCTGCTCTC
CCGACTACTGGGCCAGCATTACGGCCTTACACTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACTTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGCATCCGGAGAAACGATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13529.45 Da
• Isoelectric Point: 6.6290

>AT50625 WP_040089920.1 NZ_CP010133:c3244061-3243693 [Escherichia coli]
VSDTLPGTILPDDNHDRLWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLGSGGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT50625 NZ_CP010133:c3244061-3243693 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAATACTTCCCGACGACAATCACGACCGCCTCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGTCTGACCTGCAAAGCTGACACTCTCGGTAGCGG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA