Detailed information of TA system TA050623

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 2482421..2483253
• Replicon: chromosome
• Accession: NZ_CP010133
• Organism: Escherichia coli strain C11

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: RG33_RS12720
• Protein ID: WP_040076080.1
• Coordinates: 2482421..2482798 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: RG33_RS12725
• Protein ID: WP_040076083.1
• Coordinates: 2482888..2483253 (-)
• Length: 122 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RG33_RS12695	2478061..2478984	-	924	WP_000535960.1	carboxylate/amino acid/amine transporter	-
RG33_RS12700	2479095..2480279	-	1185	WP_001172876.1	sugar efflux transporter	-
RG33_RS12710	2481075..2481917	-	843	WP_040090283.1	DUF4942 domain-containing protein	-
RG33_RS28775	2482002..2482199	-	198	WP_085948736.1	DUF957 domain-containing protein	-
RG33_RS28780	2482275..2482424	-	150	Protein_2250	hypothetical protein	-
RG33_RS12720	2482421..2482798	-	378	WP_040076080.1	TA system toxin CbtA family protein	Toxin
RG33_RS12725	2482888..2483253	-	366	WP_040076083.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
RG33_RS12730	2483331..2483552	-	222	WP_000692300.1	DUF987 domain-containing protein	-
RG33_RS12735	2483615..2484091	-	477	WP_001186192.1	RadC family protein	-
RG33_RS12740	2484106..2484591	-	486	WP_000206670.1	antirestriction protein	-
RG33_RS12745	2484683..2485501	-	819	WP_001175163.1	DUF945 domain-containing protein	-
RG33_RS12750	2485591..2485824	-	234	WP_001278287.1	DUF905 family protein	-
RG33_RS12755	2485830..2486507	-	678	WP_040076088.1	hypothetical protein	-
RG33_RS12760	2486655..2487335	-	681	WP_001282919.1	WYL domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (17-116)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13987.82 Da
• Isoelectric Point: 6.9669

>T50623 WP_040076080.1 NZ_CP010133:c2482798-2482421 [Escherichia coli]
MNTLPDTHIREASHCQSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGTSSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPGAKQ

Nucleotide

• Length: 378 bp

>T50623 NZ_CP010133:c2482798-2482421 [Escherichia coli]
ATGAACACATTACCCGATACACACATACGGGAGGCATCGCATTGCCAGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCATTACGGCCTCACACTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCACTGGTGCGTACCGACCAGCCGGGATTC
AGCGCAGGAACTTCGTCGCAGTTAATAAACAGTATTGATATCCTCCGGGCACGCCGGGCAACCGGCCTGATGACCCGCGA
CAACTACAGAACGGTAAATAACATTACCCTGGGTAAACATCCGGGGGCGAAACAATGA

Antitoxin

Protein

• Length: 122 a.a.
• Molecular weight: 13649.57 Da
• Isoelectric Point: 7.2621

>AT50623 WP_040076083.1 NZ_CP010133:c2483253-2482888 [Escherichia coli]
VSDTLPGTTYPDNNNRPWWGLPCTVMPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSGE
LNPRHQHTVTLYAKRLTCEADTLGSCGYVYMAVYPTPETKK

Nucleotide

• Length: 366 bp

>AT50623 NZ_CP010133:c2483253-2482888 [Escherichia coli]
GTGTCAGACACACTGCCCGGGACAACGTATCCCGACAACAACAACCGCCCCTGGTGGGGGCTACCCTGCACCGTGATGCC
CTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGCTTGCATTACCTTGCAGACCGCGCCGGTATCAGAGGCCGGTTCA
GCGACGCGGATGCGTACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGTGAA
CTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAAGGCTGACCTGCGAAGCCGACACCCTCGGCAGTTGTGG
CTACGTTTATATGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA