Detailed information of TA system TA050619

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 1240794..1241595
• Replicon: chromosome
• Accession: NZ_CP010133
• Organism: Escherichia coli strain C11

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: RG33_RS06325
• Protein ID: WP_001094440.1
• Coordinates: 1240794..1241171 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: RG33_RS06330
• Protein ID: WP_024166067.1
• Coordinates: 1241218..1241595 (-)
• Length: 126 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RG33_RS06300	1236324..1237643	+	1320	WP_000144687.1	site-specific integrase	-
RG33_RS06305	1237736..1238584	-	849	WP_001280444.1	DUF4942 domain-containing protein	-
RG33_RS06310	1238867..1239886	-	1020	WP_000875212.1	IS110 family transposase	-
RG33_RS06315	1240100..1240297	-	198	WP_000839232.1	DUF957 domain-containing protein	-
RG33_RS06320	1240309..1240797	-	489	WP_000761702.1	hypothetical protein	-
RG33_RS06325	1240794..1241171	-	378	WP_001094440.1	TA system toxin CbtA family protein	Toxin
RG33_RS06330	1241218..1241595	-	378	WP_024166067.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
RG33_RS06335	1241670..1241891	-	222	WP_000692350.1	DUF987 domain-containing protein	-
RG33_RS06340	1241978..1242454	-	477	WP_001367144.1	RadC family protein	-
RG33_RS06345	1242470..1242949	-	480	WP_001449312.1	antirestriction protein	-
RG33_RS06350	1243043..1243288	-	246	WP_000680583.1	hypothetical protein	-
RG33_RS06355	1243288..1244106	-	819	WP_001234621.1	DUF945 domain-containing protein	-
RG33_RS06365	1244327..1244737	-	411	WP_000846706.1	hypothetical protein	-
RG33_RS06370	1244753..1245436	-	684	WP_000775504.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	1238867..1239886	1019

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13961.87 Da
• Isoelectric Point: 7.3225

>T50619 WP_001094440.1 NZ_CP010133:c1241171-1240794 [Escherichia coli]
MNTLPDTHVREASGCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGAPSQLINSIDILRARRATGLMTRSHYRTVNDITLGKRTEAKR

Nucleotide

• Length: 378 bp

>T50619 NZ_CP010133:c1241171-1240794 [Escherichia coli]
ATGAATACATTACCCGACACTCACGTACGGGAGGCATCGGGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTATGGTCTCACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGATGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGAACCGACCAGCCGGGATTC
AGCGCAGGAGCCCCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCCCGCCGGGCAACCGGCCTGATGACCCGCAG
CCACTACAGAACGGTAAATGACATTACCCTGGGTAAACGCACGGAGGCAAAACGATGA

Antitoxin

Protein

• Length: 126 a.a.
• Molecular weight: 13676.53 Da
• Isoelectric Point: 5.9505

>AT50619 WP_024166067.1 NZ_CP010133:c1241595-1241218 [Escherichia coli]
VSDALSGTMLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTGG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPAAPATTV

Nucleotide

• Length: 378 bp

>AT50619 NZ_CP010133:c1241595-1241218 [Escherichia coli]
GTGTCAGACGCACTCTCCGGGACAATGCTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCCCGACTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCAGGT
TCAGCGACGCGGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCGGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACACTGTATGCGAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCGGTTTATCCGACACCGGCAGCGCCCGCAACCACCGTATAA