Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 4853906..4854560 Replicon chromosome
Accession NZ_CP010132
Organism Escherichia coli strain C10

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag RG32_RS25195 Protein ID WP_000244777.1
Coordinates 4853906..4854313 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag RG32_RS25200 Protein ID WP_000354046.1
Coordinates 4854294..4854560 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RG32_RS25175 4849863..4851596 - 1734 WP_000813212.1 single-stranded-DNA-specific exonuclease RecJ -
RG32_RS25180 4851602..4852312 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
RG32_RS25185 4852337..4853233 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
RG32_RS25190 4853345..4853866 + 522 WP_001055874.1 flavodoxin FldB -
RG32_RS25195 4853906..4854313 - 408 WP_000244777.1 toxin CptA Toxin
RG32_RS25200 4854294..4854560 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
RG32_RS25205 4854803..4855783 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
RG32_RS25210 4855979..4856638 - 660 WP_000250274.1 hemolysin III family protein -
RG32_RS25215 4856802..4857113 - 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
RG32_RS25220 4857158..4858591 + 1434 WP_001307385.1 6-phospho-beta-glucosidase BglA -
RG32_RS25225 4858648..4859391 - 744 WP_000951964.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T50617 WP_000244777.1 NZ_CP010132:c4854313-4853906 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T50617 NZ_CP010132:c4854313-4853906 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT50617 WP_000354046.1 NZ_CP010132:c4854560-4854294 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT50617 NZ_CP010132:c4854560-4854294 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References