Detailed information of TA system TA050605

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 3433234..3433760
• Replicon: chromosome
• Accession: NZ_CP010132
• Organism: Escherichia coli strain C10

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1EXL1
• Locus tag: RG32_RS17810
• Protein ID: WP_000323025.1
• Coordinates: 3433234..3433521 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: S1F6D3
• Locus tag: RG32_RS17815
• Protein ID: WP_000534858.1
• Coordinates: 3433521..3433760 (-)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RG32_RS17770	3428257..3428472	-	216	WP_000839590.1	phage lysis protein EssD	-
RG32_RS27315	3428692..3428862	+	171	WP_211180497.1	putative zinc-binding protein YnfU	-
RG32_RS17775	3429226..3429441	-	216	WP_000066484.1	cold shock-like protein CspB	-
RG32_RS17780	3429742..3429954	+	213	WP_000087756.1	cold shock-like protein CspF	-
RG32_RS26850	3430009..3430098	+	90	WP_120795389.1	hypothetical protein	-
RG32_RS17785	3430377..3431129	-	753	WP_040090316.1	antitermination protein	-
RG32_RS17790	3431143..3432192	-	1050	WP_001265198.1	DUF968 domain-containing protein	-
RG32_RS17795	3432194..3432472	-	279	WP_040090315.1	hypothetical protein	-
RG32_RS17800	3432539..3432790	-	252	WP_000980994.1	hypothetical protein	-
RG32_RS17805	3433007..3433162	-	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	-
RG32_RS17810	3433234..3433521	-	288	WP_000323025.1	type II toxin-antitoxin system mRNA interferase RelE	Toxin
RG32_RS17815	3433521..3433760	-	240	WP_000534858.1	type II toxin-antitoxin system antitoxin RelB	Antitoxin
RG32_RS17820	3433785..3434090	+	306	WP_001326990.1	hypothetical protein	-
RG32_RS17830	3434293..3434625	+	333	WP_001317460.1	FlxA-like family protein	-
RG32_RS17835	3435062..3436403	-	1342	Protein_3209	ISNCY family transposase	-
RG32_RS27320	3437172..3437459	-	288	Protein_3210	hypothetical protein	-
RG32_RS17860	3438070..3438426	-	357	WP_001374839.1	class I SAM-dependent methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3396661..3458625	61964

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-79)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11225.22 Da
• Isoelectric Point: 10.1967

>T50605 WP_000323025.1 NZ_CP010132:c3433521-3433234 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Nucleotide

• Length: 288 bp

>T50605 NZ_CP010132:c3433521-3433234 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9071.48 Da
• Isoelectric Point: 4.5230

>AT50605 WP_000534858.1 NZ_CP010132:c3433760-3433521 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Nucleotide

• Length: 240 bp

>AT50605 NZ_CP010132:c3433760-3433521 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Structures

Toxin

• PDB: 4V7K
• PDB: 4V7J
• PDB: 4FXI
• PDB: 4FXE
• PDB: 2KC8
• PDB: 4FXH
• PDB: 2KC9

Antitoxin

• PDB: 4FXE
• PDB: 2KC8
• PDB: 2K29
• AlphaFold DB: A0A4V1CTS8