Detailed information of TA system TA050595

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 2046583..2047384
• Replicon: chromosome
• Accession: NZ_CP010132
• Organism: Escherichia coli strain C10

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: RG32_RS10625
• Protein ID: WP_001094440.1
• Coordinates: 2047007..2047384 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: RG32_RS10620
• Protein ID: WP_024166067.1
• Coordinates: 2046583..2046960 (+)
• Length: 126 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RG32_RS10580	2042742..2043425	+	684	WP_000775504.1	hypothetical protein	-
RG32_RS10585	2043441..2043851	+	411	WP_000846706.1	hypothetical protein	-
RG32_RS10595	2044072..2044890	+	819	WP_001234621.1	DUF945 domain-containing protein	-
RG32_RS10600	2044890..2045135	+	246	WP_000680583.1	hypothetical protein	-
RG32_RS10605	2045229..2045708	+	480	WP_001449312.1	antirestriction protein	-
RG32_RS10610	2045724..2046200	+	477	WP_001367144.1	RadC family protein	-
RG32_RS10615	2046287..2046508	+	222	WP_000692350.1	DUF987 domain-containing protein	-
RG32_RS10620	2046583..2046960	+	378	WP_024166067.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
RG32_RS10625	2047007..2047384	+	378	WP_001094440.1	TA system toxin CbtA family protein	Toxin
RG32_RS10630	2047381..2047869	+	489	WP_000761702.1	hypothetical protein	-
RG32_RS10635	2047881..2048078	+	198	WP_000839232.1	DUF957 domain-containing protein	-
RG32_RS10640	2048292..2049311	+	1020	WP_000875212.1	IS110 family transposase	-
RG32_RS10645	2049594..2050442	+	849	WP_001280444.1	DUF4942 domain-containing protein	-
RG32_RS10650	2050535..2051854	-	1320	WP_000144687.1	site-specific integrase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2010451..2058416	47965
	-	flank	IS/Tn	-	-	2048292..2049311	1019

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13961.87 Da
• Isoelectric Point: 7.3225

>T50595 WP_001094440.1 NZ_CP010132:2047007-2047384 [Escherichia coli]
MNTLPDTHVREASGCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGAPSQLINSIDILRARRATGLMTRSHYRTVNDITLGKRTEAKR

Nucleotide

• Length: 378 bp

>T50595 NZ_CP010132:2047007-2047384 [Escherichia coli]
ATGAATACATTACCCGACACTCACGTACGGGAGGCATCGGGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTATGGTCTCACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGATGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGAACCGACCAGCCGGGATTC
AGCGCAGGAGCCCCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCCCGCCGGGCAACCGGCCTGATGACCCGCAG
CCACTACAGAACGGTAAATGACATTACCCTGGGTAAACGCACGGAGGCAAAACGATGA

Antitoxin

Protein

• Length: 126 a.a.
• Molecular weight: 13676.53 Da
• Isoelectric Point: 5.9505

>AT50595 WP_024166067.1 NZ_CP010132:2046583-2046960 [Escherichia coli]
VSDALSGTMLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTGG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPAAPATTV

Nucleotide

• Length: 378 bp

>AT50595 NZ_CP010132:2046583-2046960 [Escherichia coli]
GTGTCAGACGCACTCTCCGGGACAATGCTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCCCGACTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCAGGT
TCAGCGACGCGGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCGGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACACTGTATGCGAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCGGTTTATCCGACACCGGCAGCGCCCGCAACCACCGTATAA