Detailed information of TA system TA050589

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 43716..44548
• Replicon: chromosome
• Accession: NZ_CP010132
• Organism: Escherichia coli strain C10

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: RG32_RS00295
• Protein ID: WP_073487848.1
• Coordinates: 44174..44548 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: RG32_RS00290
• Protein ID: WP_040089920.1
• Coordinates: 43716..44084 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RG32_RS00255	40635..41090	+	456	WP_000581504.1	hypothetical protein	-
RG32_RS00260	41169..41402	+	234	WP_001119729.1	DUF905 family protein	-
RG32_RS00270	41502..42320	+	819	WP_001234620.1	DUF945 domain-containing protein	-
RG32_RS00275	42375..42860	+	486	WP_000849582.1	antirestriction protein	-
RG32_RS00280	42876..43352	+	477	WP_001186726.1	RadC family protein	-
RG32_RS00285	43415..43636	+	222	WP_000692323.1	DUF987 domain-containing protein	-
RG32_RS00290	43716..44084	+	369	WP_040089920.1	type IV toxin-antitoxin system toxin CbtA	Antitoxin
RG32_RS00295	44174..44548	+	375	WP_073487848.1	TA system toxin CbtA family protein	Toxin
RG32_RS00300	44545..45033	+	489	WP_000777541.1	hypothetical protein	-
RG32_RS00305	45045..45242	+	198	WP_000839281.1	DUF957 domain-containing protein	-
RG32_RS00310	45339..45911	+	573	WP_001290246.1	DUF4942 domain-containing protein	-
RG32_RS00315	46192..46728	-	537	WP_000942770.1	GspM family type II secretion system protein YghD	-
RG32_RS00320	46730..47908	-	1179	WP_000094972.1	type II secretion system protein GspL	-
RG32_RS00325	47905..48882	-	978	WP_000633209.1	type II secretion system minor pseudopilin GspK	-
RG32_RS00330	48879..49484	-	606	WP_001255030.1	type II secretion system minor pseudopilin GspJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (19-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 14004.02 Da
• Isoelectric Point: 8.7453

>T50589 WP_073487848.1 NZ_CP010132:44174-44548 [Escherichia coli]
MKTLPNTHVREASSCPSPVTIWQTLLSRLLGQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEKR

Nucleotide

• Length: 375 bp

>T50589 NZ_CP010132:44174-44548 [Escherichia coli]
ATGAAAACATTACCCAATACACACGTACGGGAGGCATCGAGCTGCCCGTCTCCCGTCACCATCTGGCAGACTCTGCTCTC
CCGACTACTGGGCCAGCATTACGGCCTTACACTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACTTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGCATCCGGAGAAACGATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13529.45 Da
• Isoelectric Point: 6.6290

>AT50589 WP_040089920.1 NZ_CP010132:43716-44084 [Escherichia coli]
VSDTLPGTILPDDNHDRLWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLGSGGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT50589 NZ_CP010132:43716-44084 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAATACTTCCCGACGACAATCACGACCGCCTCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGTCTGACCTGCAAAGCTGACACTCTCGGTAGCGG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA