Detailed information of TA system TA050588

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-Phd
• Location: 5538..6139
• Replicon: plasmid B
• Accession: NZ_CP010131
• Organism: Escherichia coli strain C9

Toxin (Protein)

• Gene name: doc
• Uniprot ID: A0A5U0JPI4
• Locus tag: RG31_RS24945
• Protein ID: WP_024166788.1
• Coordinates: 5538..5918 (-)
• Length: 127 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: U9YQH9
• Locus tag: RG31_RS24950
• Protein ID: WP_001190712.1
• Coordinates: 5918..6139 (-)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RG31_RS24915	785..1822	-	1038	WP_048659773.1	hypothetical protein	-
RG31_RS24920	2220..3248	+	1029	WP_048659775.1	tyrosine-type recombinase/integrase	-
RG31_RS24925	3321..3665	+	345	WP_004010537.1	hypothetical protein	-
RG31_RS24930	3662..4153	+	492	WP_004010538.1	hypothetical protein	-
RG31_RS24935	4146..4640	+	495	WP_048659776.1	dUTP diphosphatase	-
RG31_RS24940	4654..5454	+	801	WP_001743161.1	hypothetical protein	-
RG31_RS24945	5538..5918	-	381	WP_024166788.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
RG31_RS24950	5918..6139	-	222	WP_001190712.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
RG31_RS24955	6212..6604	-	393	WP_000506718.1	hypothetical protein	-
RG31_RS24960	6716..6964	-	249	WP_001286530.1	DNA polymerase III subunit theta	-
RG31_RS24965	6967..7167	-	201	WP_001408980.1	hypothetical protein	-
RG31_RS24970	7303..7542	-	240	Protein_12	hypothetical protein	-
RG31_RS24975	7590..7787	-	198	WP_048659784.1	DUF4752 family protein	-
RG31_RS24980	7746..8411	+	666	Protein_14	IS5 family transposase	-
RG31_RS24985	8519..8707	+	189	WP_000957857.1	hypothetical protein	-
RG31_RS24990	8717..9916	+	1200	WP_000948429.1	IS91 family transposase	-
RG31_RS24995	10089..10262	+	174	Protein_17	ATP-dependent helicase	-
RG31_RS25000	10265..10543	+	279	WP_001711179.1	hypothetical protein	-
RG31_RS25005	10603..11025	+	423	WP_024172705.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	tet(A) / qnrS1	-	1..56460	56460

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Fido domain (4-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-51)

Sequences

Toxin

Protein

• Length: 127 a.a.
• Molecular weight: 13663.58 Da
• Isoelectric Point: 5.6406

>T50588 WP_024166788.1 NZ_CP010131:c5918-5538 [Escherichia coli]
MRHISPEELIALHDANISRYGGLPGMSDPGRAEAIIGRVQARVVYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGIQVYDSPVLVELAVGAATGEIPVSSVAEKLRELFGSNI

Nucleotide

• Length: 381 bp

>T50588 NZ_CP010131:c5918-5538 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTGATAGCGCTTCATGATGCGAATATAAGCCGCTACGGCGGCCTGCCGGGAATGTC
AGATCCGGGCAGGGCAGAGGCCATTATCGGGAGAGTCCAGGCCAGAGTTGTGTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACGGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGCTATTTCTACGCCGTAACGGTATACAGGTATATGATTCTCCCGTGCTGGTGGAACTTGCGGTGGGGGCCGCAACTGG
TGAAATCCCCGTATCTTCAGTAGCGGAAAAACTACGTGAATTATTTGGTTCCAATATTTGA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8133.10 Da
• Isoelectric Point: 4.7708

>AT50588 WP_001190712.1 NZ_CP010131:c6139-5918 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Nucleotide

• Length: 222 bp

>AT50588 NZ_CP010131:c6139-5918 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGTGGCAACCTTTCTGAAGTGCTCAACAATGTTGAGGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Structures

Toxin

• AlphaFold DB: A0A5U0JPI4

Antitoxin

• AlphaFold DB: A0A829CJB6