Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2495856..2496494 Replicon chromosome
Accession NZ_CP010129
Organism Escherichia coli strain C9

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag RG31_RS13380 Protein ID WP_000813794.1
Coordinates 2496318..2496494 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag RG31_RS13375 Protein ID WP_001270286.1
Coordinates 2495856..2496272 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RG31_RS13355 2491008..2491949 - 942 WP_001251304.1 ABC transporter permease -
RG31_RS13360 2491950..2492963 - 1014 WP_073510954.1 ABC transporter ATP-binding protein -
RG31_RS13365 2492981..2494126 - 1146 WP_000047424.1 ABC transporter substrate-binding protein -
RG31_RS13370 2494371..2495777 - 1407 WP_000760626.1 PLP-dependent aminotransferase family protein -
RG31_RS13375 2495856..2496272 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
RG31_RS13380 2496318..2496494 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
RG31_RS13385 2496716..2496946 + 231 WP_000494244.1 YncJ family protein -
RG31_RS13390 2497038..2498999 - 1962 WP_001303492.1 23S rRNA 5-hydroxycytidine C2501 synthase -
RG31_RS13395 2499072..2499608 - 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
RG31_RS13400 2499661..2500875 + 1215 WP_001326689.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T50576 WP_000813794.1 NZ_CP010129:c2496494-2496318 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T50576 NZ_CP010129:c2496494-2496318 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT50576 WP_001270286.1 NZ_CP010129:c2496272-2495856 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT50576 NZ_CP010129:c2496272-2495856 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References