Detailed information of TA system TA050560

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-DnaT
• Location: 34185..34707
• Replicon: plasmid B
• Accession: NZ_CP010127
• Organism: Escherichia coli strain C8

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: RG30_RS26470
• Protein ID: WP_073534379.1
• Coordinates: 34426..34707 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: RG30_RS26465
• Protein ID: WP_073534378.1
• Coordinates: 34185..34436 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RG30_RS27785	29643..29828	+	186	Protein_29	YcxB family protein	-
RG30_RS26435	30004..30237	+	234	WP_073534387.1	YcxB family protein	-
RG30_RS26440	30255..31115	+	861	WP_073534376.1	hypothetical protein	-
RG30_RS26445	31137..31562	-	426	WP_149026027.1	DNA distortion polypeptide 3	-
RG30_RS26450	31630..32076	-	447	WP_073534377.1	hypothetical protein	-
RG30_RS26455	32116..32952	-	837	WP_001802123.1	replication initiation protein	-
RG30_RS26465	34185..34436	+	252	WP_073534378.1	plasmid stabilization protein	Antitoxin
RG30_RS26470	34426..34707	+	282	WP_073534379.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
RG30_RS26475	34853..35176	+	324	WP_001181903.1	hypothetical protein	-
RG30_RS26480	35221..35466	+	246	WP_001550454.1	hypothetical protein	-
RG30_RS26485	35456..35671	+	216	WP_001180116.1	hypothetical protein	-
RG30_RS27550	35764..36092	+	329	Protein_40	hypothetical protein	-
RG30_RS27790	36135..36305	+	171	WP_182487779.1	hypothetical protein	-
RG30_RS27795	36383..36532	+	150	WP_175154170.1	hypothetical protein	-
RG30_RS26495	36545..36817	+	273	WP_000167280.1	hypothetical protein	-
RG30_RS26500	37133..37678	+	546	WP_073534381.1	DNA distortion polypeptide 1	-
RG30_RS26505	37681..38847	+	1167	WP_073534382.1	relaxase/mobilization nuclease domain-containing protein	-
RG30_RS26510	39200..39685	+	486	WP_073534383.1	transcription termination factor NusG	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..42655	42655

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 11021.83 Da
• Isoelectric Point: 10.5938

>T50560 WP_073534379.1 NZ_CP010127:34426-34707 [Escherichia coli]
MTYELAFDRRALKEWQKLGHTIREQFKKKLAERLENPRVPSARLHGHADRYKIKLRASGYRLVYQVIDEKVVLLVIAVGR
RESSEVYQIADLR

Nucleotide

• Length: 282 bp

>T50560 NZ_CP010127:34426-34707 [Escherichia coli]
ATGACCTATGAACTGGCTTTTGACCGTAGAGCACTGAAGGAATGGCAGAAACTCGGCCACACCATCCGTGAACAATTCAA
AAAGAAACTGGCAGAACGGCTGGAAAATCCACGCGTACCCTCAGCCCGGTTACATGGTCATGCTGATCGCTATAAAATCA
AACTTCGTGCATCTGGCTACAGACTTGTATATCAAGTCATTGATGAGAAAGTCGTTTTACTCGTTATTGCCGTTGGAAGA
AGGGAAAGCAGCGAAGTCTATCAGATCGCAGATTTGCGCTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9222.48 Da
• Isoelectric Point: 4.1683

>AT50560 WP_073534378.1 NZ_CP010127:34185-34436 [Escherichia coli]
MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCVPPKLYAYYRELAEDAELNAVVDERMKNPEIVKVNL
DDL

Nucleotide

• Length: 252 bp

>AT50560 NZ_CP010127:34185-34436 [Escherichia coli]
ATGAGCTATCAAATTCTGACAACCACAGCGGCCAGTATTACTGACCTGAAAAAAAATCCTATGGGAACCGTAGCTGAAGG
TGAAGGGGACGCTGTTGCGATCCTGAACCGAAACGAACCGGCGTTCTATTGCGTTCCACCAAAACTTTACGCCTACTATC
GGGAACTCGCTGAAGATGCTGAGTTAAACGCTGTTGTTGATGAGCGCATGAAAAACCCGGAAATTGTGAAGGTTAACCTG
GATGACCTATGA