Detailed information of TA system TA050559

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/ElaA-DUF1778
• Location: 129876..130636
• Replicon: plasmid A
• Accession: NZ_CP010126
• Organism: Escherichia coli strain C8

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: A0A8H9BF00
• Locus tag: RG30_RS26195
• Protein ID: WP_062859951.1
• Coordinates: 130148..130636 (+)
• Length: 163 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: -
• Locus tag: RG30_RS26190
• Protein ID: WP_073534343.1
• Coordinates: 129876..130160 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RG30_RS26125	124902..125363	+	462	WP_073534337.1	thermonuclease family protein	-
RG30_RS26130	125409..125618	+	210	WP_001299729.1	hemolysin expression modulator Hha	-
RG30_RS26135	125657..126247	+	591	WP_073534338.1	DUF2726 domain-containing protein	-
RG30_RS26145	126402..126866	+	465	WP_073534339.1	hypothetical protein	-
-	127018..127079	-	62	NuclAT_1	-	-
-	127018..127079	-	62	NuclAT_1	-	-
-	127018..127079	-	62	NuclAT_1	-	-
-	127018..127079	-	62	NuclAT_1	-	-
RG30_RS26155	127066..127272	+	207	WP_001362723.1	type I toxin-antitoxin system Hok family toxin	-
RG30_RS26160	127556..127813	+	258	WP_073534340.1	replication regulatory protein RepA	-
RG30_RS26170	128035..128109	+	75	WP_032144212.1	RepA leader peptide Tap	-
RG30_RS26175	128102..128959	+	858	WP_073534341.1	incFII family plasmid replication initiator RepA	-
RG30_RS27765	129661..129822	+	162	WP_073534342.1	hypothetical protein	-
RG30_RS26190	129876..130160	+	285	WP_073534343.1	DUF1778 domain-containing protein	Antitoxin
RG30_RS26195	130148..130636	+	489	WP_062859951.1	GNAT family N-acetyltransferase	Toxin
RG30_RS27770	131013..131288	-	276	WP_157925946.1	hypothetical protein	-
RG30_RS26210	131682..132317	+	636	WP_073534345.1	proQ/FINO family protein	-
RG30_RS26225	133276..134136	-	861	WP_073534348.1	alpha/beta hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	vat / paa / papH / papC / papD	1..143617	143617

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (27-152)

Antitoxin

• Vibrio phage ICP1, Orf50 (12-88)

Sequences

Toxin

Protein

• Length: 163 a.a.
• Molecular weight: 17614.34 Da
• Isoelectric Point: 9.8566

>T50559 WP_062859951.1 NZ_CP010126:130148-130636 [Escherichia coli]
MGGVTAPEPLSSFHQAAEFVSGEAVLDDWLKQKGLKNQALGATRTFVVCRKGSQQIVGFYSLATGSVNHTEAAGNLRRNM
PDPIPVIILARLAVDVSFRGKGLGADLLHDAVCRCYRVAENIGVRAIMVHALTENAKQFYIHHGFKPSKSQEQTLFLKLP
GK

Nucleotide

• Length: 489 bp

>T50559 NZ_CP010126:130148-130636 [Escherichia coli]
ATGGGAGGTGTAACAGCACCAGAACCTTTGTCCTCATTTCATCAGGCCGCTGAATTTGTCAGTGGTGAAGCTGTGCTTGA
TGACTGGTTAAAGCAGAAGGGGCTGAAAAATCAGGCTCTCGGAGCCACACGGACTTTTGTTGTATGCAGAAAAGGTTCGC
AACAAATAGTCGGTTTTTACTCTCTGGCCACCGGGAGTGTTAATCATACAGAGGCTGCAGGCAACCTTCGGCGCAATATG
CCAGACCCTATACCTGTTATTATACTTGCACGCCTGGCCGTTGATGTGTCATTCCGTGGAAAAGGGCTTGGTGCAGATTT
ACTTCATGATGCTGTTTGTCGTTGTTACAGGGTTGCCGAAAATATCGGTGTGCGTGCAATTATGGTTCATGCGCTTACGG
AAAACGCAAAACAGTTTTATATTCATCATGGTTTTAAGCCATCAAAAAGCCAGGAGCAAACATTGTTTCTTAAACTCCCC
GGCAAGTAA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10947.62 Da
• Isoelectric Point: 4.7037

>AT50559 WP_073534343.1 NZ_CP010126:129876-130160 [Escherichia coli]
MIYGGFMKSGVQLNLRARESQRILIDTAAEILHKSRTDFILEMACKAAEDIILDRRVFNFNDRQYEEFIEMLDAPVADET
AIEKLLARKPQWEV

Nucleotide

• Length: 285 bp

>AT50559 NZ_CP010126:129876-130160 [Escherichia coli]
ATGATATACGGAGGCTTTATGAAATCAGGTGTTCAGCTCAATCTCAGGGCAAGAGAATCTCAGCGGATACTCATTGATAC
CGCTGCAGAAATTCTTCATAAGTCGCGTACAGACTTCATTCTGGAAATGGCCTGTAAAGCTGCTGAAGATATTATTCTTG
ACCGGCGGGTGTTTAATTTTAATGACAGGCAATATGAAGAGTTCATCGAAATGCTTGATGCTCCGGTTGCTGATGAGACT
GCCATCGAAAAACTGCTGGCGAGGAAACCTCAATGGGAGGTGTAA