Detailed information of TA system TA050558

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 116961..117586
• Replicon: plasmid A
• Accession: NZ_CP010126
• Organism: Escherichia coli strain C8

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A828AN07
• Locus tag: RG30_RS26095
• Protein ID: WP_059257082.1
• Coordinates: 116961..117359 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A8H9EG71
• Locus tag: RG30_RS26100
• Protein ID: WP_000450526.1
• Coordinates: 117359..117586 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RG30_RS26075	112459..112965	+	507	WP_073534330.1	conjugal transfer entry exclusion protein TraS	-
RG30_RS26080	113020..113733	+	714	WP_077899822.1	complement resistance protein TraT	-
RG30_RS26085	113936..114676	+	741	WP_073534332.1	hypothetical protein	-
RG30_RS26090	114727..116952	+	2226	WP_073534333.1	type IV conjugative transfer system coupling protein TraD	-
RG30_RS26095	116961..117359	-	399	WP_059257082.1	tRNA(fMet)-specific endonuclease VapC	Toxin
RG30_RS26100	117359..117586	-	228	WP_000450526.1	toxin-antitoxin system antitoxin VapB	Antitoxin

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	vat / paa / papH / papC / papD	1..143617	143617

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-51)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14945.27 Da
• Isoelectric Point: 7.8605

>T50558 WP_059257082.1 NZ_CP010126:c117359-116961 [Escherichia coli]
MLKFMLDTNICIFTIKNKPASVRERFNLNQGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLDVLDYDAAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNIREFERMDGLRIEDWS

Nucleotide

• Length: 399 bp

>T50558 NZ_CP010126:c117359-116961 [Escherichia coli]
ATGCTGAAGTTTATGCTCGATACCAACATCTGCATTTTTACGATAAAGAACAAACCCGCCAGTGTCAGGGAACGTTTTAA
CCTGAATCAGGGGAGAATGTGTATCAGTTCGGTCACCCTGATGGAGCTGATATATGGTGCAGAAAAAAGCCAGATGCCTG
AACGTAATCTCGCTGTGATCGAGGGATTTGTTTCCCGCCTTGATGTTCTGGATTACGACGCCGCTGCAGCCACACATACC
GGCCAGATAAGGGCAGAACTTGCCCGTCAGGGACGTCCTGTCGGACCATTTGATCAAATGATCGCAGGCCATGCCCGCAG
CCGGGGGCTGATAGTTGTGACTAACAACATCCGGGAATTTGAACGTATGGACGGTCTGAGAATTGAAGACTGGAGCTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.53 Da
• Isoelectric Point: 4.5358

>AT50558 WP_000450526.1 NZ_CP010126:c117586-117359 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAGESWDEWFDGNSVSADFMDNREQPGMQERESF

Nucleotide

• Length: 228 bp

>AT50558 NZ_CP010126:c117586-117359 [Escherichia coli]
ATGGAAACCACCGTATTTCTCAGCAACCGCAGCCAGGCGGTCAGACTGCCAAAAGCGGTTGCATTGCCGGAAAACGTAAA
ACGCGTTGAAGTGATTGCTGTCGGACGAACCCGTATCATTACGCCAGCCGGAGAATCATGGGATGAATGGTTCGACGGAA
ACAGCGTCAGCGCCGATTTTATGGATAACCGGGAACAGCCCGGCATGCAGGAAAGGGAGTCATTCTGA

Structures

Toxin

• AlphaFold DB: A0A828AN07