Detailed information of TA system TA050552

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 37007..37532
• Replicon: plasmid A
• Accession: NZ_CP010126
• Organism: Escherichia coli strain C8

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: A0A8S7NRU4
• Locus tag: RG30_RS25525
• Protein ID: WP_001159873.1
• Coordinates: 37007..37312 (-)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: Q3ZU16
• Locus tag: RG30_RS25530
• Protein ID: WP_000813639.1
• Coordinates: 37314..37532 (-)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RG30_RS25495	32480..34015	+	1536	WP_073534282.1	pore-forming bacteriocin colicin B	-
RG30_RS25500	34033..34560	-	528	WP_000203274.1	colicin B immunity protein	-
RG30_RS25510	34806..35621	+	816	WP_001382745.1	lipid II-degrading bacteriocin colicin M	-
RG30_RS25515	35671..36024	-	354	WP_000864816.1	colicin M immunity protein	-
RG30_RS25520	36197..37006	-	810	WP_000016972.1	site-specific integrase	-
RG30_RS25525	37007..37312	-	306	WP_001159873.1	type II toxin-antitoxin system toxin CcdB	Toxin
RG30_RS25530	37314..37532	-	219	WP_000813639.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
RG30_RS25540	38126..38356	+	231	WP_001261277.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
RG30_RS25545	38353..38769	+	417	WP_077899813.1	type II toxin-antitoxin system VapC family toxin	-
RG30_RS25550	38828..39268	-	441	Protein_38	transposase family protein	-
RG30_RS27515	39440..39575	-	136	Protein_39	transposase	-
RG30_RS25555	40348..40758	-	411	WP_073534286.1	subtilase cytotoxin subunit B-like protein	-
RG30_RS25560	40868..41593	-	726	WP_073534356.1	pertussis toxin-like subunit ArtA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	vat / paa / papH / papC / papD	1..143617	143617

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11691.50 Da
• Isoelectric Point: 7.2010

>T50552 WP_001159873.1 NZ_CP010126:c37312-37007 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGNESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Nucleotide

• Length: 306 bp

>T50552 NZ_CP010126:c37312-37007 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGACATTATTGACAC
GCCAGGGCGACGGATGGTGATCCCCCTGGCCAGCGCTCGCCTGCTGTCAGACAAAGTCTCCCGTGAGCTTTACCCGGTGG
TTCATGTCGGGAATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCCGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8372.30 Da
• Isoelectric Point: 4.5203

>AT50552 WP_000813639.1 NZ_CP010126:c37532-37314 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Nucleotide

• Length: 219 bp

>AT50552 NZ_CP010126:c37532-37314 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGCTGCTCAAGGCGTATGACGTGAATATCTCCGGTCT
GGTCAGTACGACCATGCAGAACGAAGCCCGGCGACTGCGCGCTGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCAG
AGGTCGCCAGGTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Structures

Antitoxin

• AlphaFold DB: A0A7U9DIR5