Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 4282987..4283618 Replicon chromosome
Accession NZ_CP010125
Organism Escherichia coli strain C8

Toxin (Protein)


Gene name hicA Uniprot ID F4NNK6
Locus tag RG30_RS22190 Protein ID WP_001259388.1
Coordinates 4283343..4283618 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag RG30_RS22185 Protein ID WP_000593555.1
Coordinates 4282987..4283346 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RG30_RS22160 4279119..4280063 + 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -
RG30_RS22165 4280060..4280893 + 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
RG30_RS22170 4280893..4281657 + 765 WP_001136236.1 nickel import ATP-binding protein NikD -
RG30_RS22175 4281654..4282460 + 807 WP_000173666.1 nickel import ATP-binding protein NikE -
RG30_RS22180 4282466..4282867 + 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
RG30_RS22185 4282987..4283346 - 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
RG30_RS22190 4283343..4283618 - 276 WP_001259388.1 type II toxin-antitoxin system HicA family toxin Toxin
RG30_RS22195 4283691..4284815 - 1125 WP_001314210.1 ABC transporter permease -
RG30_RS22200 4284815..4287550 - 2736 WP_000149132.1 ribosome-associated ATPase/putative transporter RbbA -
RG30_RS22205 4287547..4288614 - 1068 WP_029488497.1 HlyD family efflux transporter periplasmic adaptor subunit -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10151.89 Da        Isoelectric Point: 10.7076

>T50551 WP_001259388.1 NZ_CP010125:c4283618-4283343 [Escherichia coli]
MRTKVQALRKKQKNTLDQIVKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T50551 NZ_CP010125:c4283618-4283343 [Escherichia coli]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATAGTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGCAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT50551 WP_000593555.1 NZ_CP010125:c4283346-4282987 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT50551 NZ_CP010125:c4283346-4282987 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCAGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A0FFA2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References