Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3678331..3678985 Replicon chromosome
Accession NZ_CP010125
Organism Escherichia coli strain C8

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag RG30_RS18930 Protein ID WP_000244777.1
Coordinates 3678331..3678738 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag RG30_RS18935 Protein ID WP_000354046.1
Coordinates 3678719..3678985 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RG30_RS18910 3674288..3676021 - 1734 WP_000813212.1 single-stranded-DNA-specific exonuclease RecJ -
RG30_RS18915 3676027..3676737 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
RG30_RS18920 3676762..3677658 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
RG30_RS18925 3677770..3678291 + 522 WP_001055874.1 flavodoxin FldB -
RG30_RS18930 3678331..3678738 - 408 WP_000244777.1 toxin CptA Toxin
RG30_RS18935 3678719..3678985 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
RG30_RS18940 3679228..3680208 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
RG30_RS18945 3680285..3680944 - 660 WP_073533999.1 hemolysin III family protein -
RG30_RS18950 3681108..3681419 - 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
RG30_RS18955 3681464..3682897 + 1434 WP_149026008.1 6-phospho-beta-glucosidase BglA -
RG30_RS18960 3682954..3683697 - 744 WP_073534002.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T50548 WP_000244777.1 NZ_CP010125:c3678738-3678331 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T50548 NZ_CP010125:c3678738-3678331 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT50548 WP_000354046.1 NZ_CP010125:c3678985-3678719 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT50548 NZ_CP010125:c3678985-3678719 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References