Detailed information of TA system TA050545

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 3237222..3237847
• Replicon: chromosome
• Accession: NZ_CP010125
• Organism: Escherichia coli strain C8

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: RG30_RS16710
• Protein ID: WP_000911330.1
• Coordinates: 3237222..3237620 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: RG30_RS16715
• Protein ID: WP_000450524.1
• Coordinates: 3237620..3237847 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RG30_RS16690	3233100..3233300	+	201	WP_000383836.1	YpfN family protein	-
RG30_RS16695	3233410..3234108	-	699	WP_000679823.1	esterase	-
RG30_RS16700	3234182..3236197	-	2016	WP_073533935.1	tRNA cytosine(34) acetyltransferase TmcA	-
RG30_RS16705	3236212..3237075	-	864	WP_001267498.1	neutral zinc metallopeptidase	-
RG30_RS16710	3237222..3237620	-	399	WP_000911330.1	tRNA(fMet)-specific endonuclease VapC	Toxin
RG30_RS16715	3237620..3237847	-	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
RG30_RS16720	3238001..3238714	-	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
RG30_RS16730	3238927..3239961	-	1035	WP_073533937.1	outer membrane protein assembly factor BamC	-
RG30_RS16735	3239978..3240856	-	879	WP_001311023.1	4-hydroxy-tetrahydrodipicolinate synthase	-
RG30_RS16740	3241002..3241574	+	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
RG30_RS16745	3241574..3242044	+	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T50545 WP_000911330.1 NZ_CP010125:c3237620-3237222 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T50545 NZ_CP010125:c3237620-3237222 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT50545 WP_000450524.1 NZ_CP010125:c3237847-3237620 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT50545 NZ_CP010125:c3237847-3237620 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33