Detailed information of TA system TA050526

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 72984..73519
• Replicon: plasmid B
• Accession: NZ_CP010124
• Organism: Escherichia coli strain C5

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A168S2X7
• Locus tag: RG28_RS30115
• Protein ID: WP_061358347.1
• Coordinates: 72984..73271 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A167Y5I8
• Locus tag: RG28_RS30120
• Protein ID: WP_061358348.1
• Coordinates: 73268..73519 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RG28_RS30075	69165..69494	+	330	WP_001137792.1	IS66 family insertion sequence hypothetical protein	-
RG28_RS30080	69481..69861	+	381	WP_040089119.1	IS66 family insertion sequence element accessory protein TnpB	-
RG28_RS30090	69904..71488	+	1585	Protein_84	IS66 family transposase	-
RG28_RS32035	71472..71795	-	324	WP_187655803.1	hypothetical protein	-
RG28_RS30100	71843..72034	+	192	Protein_86	helix-turn-helix transcriptional regulator	-
RG28_RS30105	72052..72330	+	279	WP_072320521.1	plasmid stabilization protein	-
RG28_RS30110	72362..72619	+	258	WP_061358346.1	hypothetical protein	-
RG28_RS30115	72984..73271	-	288	WP_061358347.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
RG28_RS30120	73268..73519	-	252	WP_061358348.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
RG28_RS32195	73793..74017	+	225	Protein_91	hypothetical protein	-
RG28_RS30135	74124..74387	+	264	WP_061358349.1	hypothetical protein	-
RG28_RS30140	74377..74676	+	300	WP_000119541.1	type II toxin-antitoxin system RelE/ParE family toxin	-
RG28_RS31690	75208..75288	+	81	Protein_94	plasmid replication protein	-
RG28_RS30155	75490..76113	-	624	WP_061361454.1	potassium channel family protein	-
RG28_RS30165	76983..77990	+	1008	WP_073521408.1	hypothetical protein	-
RG28_RS30170	78060..78455	-	396	WP_061361453.1	plasmid partitioning/stability family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..86032	86032

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-83)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11074.14 Da
• Isoelectric Point: 10.5444

>T50526 WP_061358347.1 NZ_CP010124:c73271-72984 [Escherichia coli]
MTYTVKFRDDALKEWQKLDKAIQQQFAKKLKKCCENPHIPSAKLRGIKDCYKIKLRASGFRLVYQVIDAELIIAVVAVGK
RERSDVYNLASKRMR

Nucleotide

• Length: 288 bp

>T50526 NZ_CP010124:c73271-72984 [Escherichia coli]
ATGACTTACACAGTCAAATTCAGGGATGATGCGCTGAAAGAGTGGCAAAAACTGGATAAGGCTATTCAGCAGCAGTTTGC
TAAAAAGCTAAAGAAGTGCTGTGAAAATCCACATATCCCTTCAGCAAAACTGCGCGGAATAAAGGATTGCTACAAAATTA
AACTTCGTGCGTCTGGCTTCCGGCTGGTTTATCAGGTTATTGACGCTGAATTAATTATCGCTGTTGTAGCTGTAGGTAAA
CGTGAACGTAGTGACGTGTATAATCTTGCCAGCAAACGAATGAGGTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9212.49 Da
• Isoelectric Point: 4.2108

>AT50526 WP_061358348.1 NZ_CP010124:c73519-73268 [Escherichia coli]
MPNIILTDTSASVSELKKNPMATVSAGDGYPVAILNRNQPAFYCVPAELYEKMLDALDDQELVKLVAERSNQKLHDIDLD
SYL

Nucleotide

• Length: 252 bp

>AT50526 NZ_CP010124:c73519-73268 [Escherichia coli]
ATGCCAAACATCATTCTTACCGACACAAGCGCCAGTGTAAGCGAACTTAAGAAAAACCCGATGGCTACAGTCAGTGCTGG
TGATGGCTACCCAGTTGCTATTCTGAACCGCAACCAGCCCGCCTTTTACTGTGTCCCCGCAGAGCTGTACGAAAAGATGC
TCGATGCCCTGGACGATCAGGAACTGGTTAAGCTGGTCGCCGAACGCAGCAACCAGAAACTGCACGATATTGATCTGGAT
AGCTACTTATGA

Structures

Toxin

• AlphaFold DB: A0A168S2X7

Antitoxin

• AlphaFold DB: A0A167Y5I8